About: Study of Enzymatic Reaction by EMMA in a Partially Filled Capillary and Indirect or Direct Detection

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	This report describes the application of electrophoretically mediated microanalysis (EMMA) in combination with a partial filling technique and indirect or direct detection for the study of enzymes dealing with the high mobile inorganic or organic anions as substrates or products. In this set-up the part of the capillary is filled with the buffer best for the enzymatic reaction whereas the rest of the capillary with the background electrolyte optimal for separation of substrates and products. In the case of haloalkane dehalogenase, which was chosen as a model enzyme, the enzymatic reaction was performed in 20 mM glycine buffer (pH 8.6). Because of wide substrate specifity of the enzyme, it utilizes chlorinated as well as brominated substrates producing either non-absorbing chloride, or absorbing bromide ions, two different background electrolytes and the detection approaches were applied. A 10 mM chromate - 0.1 mM cetyltrimethylammonium bromide (pH 9.2) was used in combination with indirect detection a This report describes the application of electrophoretically mediated microanalysis (EMMA) in combination with a partial filling technique and indirect or direct detection for the study of enzymes dealing with the high mobile inorganic or organic anions as substrates or products. In this set-up the part of the capillary is filled with the buffer best for the enzymatic reaction whereas the rest of the capillary with the background electrolyte optimal for separation of substrates and products. In the case of haloalkane dehalogenase, which was chosen as a model enzyme, the enzymatic reaction was performed in 20 mM glycine buffer (pH 8.6). Because of wide substrate specifity of the enzyme, it utilizes chlorinated as well as brominated substrates producing either non-absorbing chloride, or absorbing bromide ions, two different background electrolytes and the detection approaches were applied. A 10 mM chromate - 0.1 mM cetyltrimethylammonium bromide (pH 9.2) was used in combination with indirect detection a (en) Jedná se o studium enzymových reakcí metodou EMMA v částečně plněné kapiláře s přímou nebo nepřímou detekcí. (cs)
Title	Study of Enzymatic Reaction by EMMA in a Partially Filled Capillary and Indirect or Direct Detection Study of Enzymatic Reaction by EMMA in a Partially Filled Capillary and Indirect or Direct Detection (en) Studium enzymových reakcí metodou EMMA v částečně plněné kapiláře s přímou nebo nepřímou detekcí. (cs)
skos:prefLabel	Study of Enzymatic Reaction by EMMA in a Partially Filled Capillary and Indirect or Direct Detection Study of Enzymatic Reaction by EMMA in a Partially Filled Capillary and Indirect or Direct Detection (en) Studium enzymových reakcí metodou EMMA v částečně plněné kapiláře s přímou nebo nepřímou detekcí. (cs)
skos:notation	RIV/00216224:14310/04:00009907!RIV09-GA0-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/03/1025), P(GA203/03/1125), Z(MSM 143100005)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Telnarová, Magdalena Vytisková, Soňa Chaloupková, Radka Glatz, Zdeněk
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	588779
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/04:00009907
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	enzymes; EMMA; indirect and direct detection (en)
http://linked.open.../riv/klicoveSlovo	enzymes indirect and direct detection EMMA
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[B0BB25DBBE67]
http://linked.open...i/riv/nazevZdroje	Electrophoresis
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Use of the real-time PCR for fetal DNA analysis in maternal plasma Application of capillary zone electrophoresis for the study of enzymes
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	25
http://linked.open...iv/tvurceVysledku	Chaloupková, Radka Glatz, Zdeněk Vytisková, Soňa Telnarová, Magdalena
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20143100005
issn	0173-0835
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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