About: In vivo kinetics of U4/U6.U5 tri-snRNP formation in Cajal bodies

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Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3038649/?tool=pubmed
Description	The U4/U6.U5 tri-small nuclear ribonucleoprotein particle (tri-snRNP) is an essential pre-mRNA splicing factor, which is assembled in a stepwise manner before each round of splicing. It was previously shown that the tri-snRNP is formed in Cajal bodies (CBs), but little is known about the dynamics of this process. Here we created a mathematical model of tri-snRNP assembly in CBs and used it to fit kinetics of individual snRNPs monitored by fluorescence recovery after photobleaching. A global fitting of all kinetic data determined key reaction constants of tri-snRNP assembly. Our model predicts that the rates of di-snRNP and tri-snRNP assemblies are similar and that approximately 230 tri-snRNPs are assembled in one CB per minute. Our analysis further indicates that tri-snRNP assembly is approximately 10-fold faster in CBs than in the surrounding nucleoplasm, which is fully consistent with the importance of CBs for snRNP formation in rapidly developing biological systems. Finally, the model predicted binding between SART3 and a CB component. We tested this prediction by Forster resonance energy transfer and revealed an interaction between SART3 and coilin in CBs. The U4/U6.U5 tri-small nuclear ribonucleoprotein particle (tri-snRNP) is an essential pre-mRNA splicing factor, which is assembled in a stepwise manner before each round of splicing. It was previously shown that the tri-snRNP is formed in Cajal bodies (CBs), but little is known about the dynamics of this process. Here we created a mathematical model of tri-snRNP assembly in CBs and used it to fit kinetics of individual snRNPs monitored by fluorescence recovery after photobleaching. A global fitting of all kinetic data determined key reaction constants of tri-snRNP assembly. Our model predicts that the rates of di-snRNP and tri-snRNP assemblies are similar and that approximately 230 tri-snRNPs are assembled in one CB per minute. Our analysis further indicates that tri-snRNP assembly is approximately 10-fold faster in CBs than in the surrounding nucleoplasm, which is fully consistent with the importance of CBs for snRNP formation in rapidly developing biological systems. Finally, the model predicted binding between SART3 and a CB component. We tested this prediction by Forster resonance energy transfer and revealed an interaction between SART3 and coilin in CBs. (en)
Title	In vivo kinetics of U4/U6.U5 tri-snRNP formation in Cajal bodies In vivo kinetics of U4/U6.U5 tri-snRNP formation in Cajal bodies (en)
skos:prefLabel	In vivo kinetics of U4/U6.U5 tri-snRNP formation in Cajal bodies In vivo kinetics of U4/U6.U5 tri-snRNP formation in Cajal bodies (en)
skos:notation	RIV/00216208:11320/11:10105570!RIV12-MSM-11320___
http://linked.open...avai/predkladatel	Matematicko-fyzikální fakulta
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GA204/07/0133), P(KAN200520801), S, Z(AV0Z50390512), Z(AV0Z50390703), Z(AV0Z50520514), Z(MSM0021620835)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Heřman, Petr Blažíková, Michaela
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Matematicko-fyzikální fakulta
http://linked.open...dnocenehoVysledku	204058
http://linked.open...ai/riv/idVysledku	RIV/00216208:11320/11:10105570
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	model; FRET; FRAP; fluorescence; Cajal bodies; formation; tri-snRNP; U4/U6.U5; kinetics (en)
http://linked.open.../riv/klicoveSlovo	FRET U4/U6.U5 tri-snRNP fluorescence formation kinetics model Cajal bodies FRAP
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[FF0107B69767]
http://linked.open...i/riv/nazevZdroje	Molecular Biology of the Cell
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Self-organization principles of non-membrane-bound organels in eukaryotic cells Targeted expression and transport of bioactive molecules
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	22
http://linked.open...iv/tvurceVysledku	Heřman, Petr Novotný, Ivan Staněk, David Blažíková, Michaela Malínský, Jan
http://linked.open...ain/vavai/riv/wos	000287305700008
http://linked.open...n/vavai/riv/zamer	Molekulární, buněčné a systémové mechanizmy závažných onemocnění lidského organizmu, jejich diagnostika, terapie a farmakoterapie New biotechnologies, nanomaterials and stem cells for use in regenerative medicine Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů Fyzika molekulárních, makromolekulárních a biologických systémů
issn	1059-1524
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1091/mbc.E10-07-0560
http://localhost/t...ganizacniJednotka	11320
is http://linked.open...avai/riv/vysledek of	In vivo kinetics of U4/U6.U5 tri-snRNP formation in Cajal bodies In vivo kinetics of U4/U6.U5 tri-snRNP formation in Cajal bodies

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