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rdf:type
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Description
| - Transgenic plants represent an excellent tool for experimental plant biology and are an important component of modern agriculture. Transgenes can be silenced long after their integration. To study the long-term changes in transgene expression in potato, the activity of two reporter genes, encoding GFP and NPTII, was monitored in a set of 17 transgenic lines over 5 years of vegetative propagation in vitro. Complete silencing of the reporter genes was observed in four lines (nearly 25 %), all of which successively silenced the two reporter genes, indicating an interconnection between their silencing. We suggest a hypothetical mechanism involving the successive silencing of the two reporter genes that involves the switch of GFP silencing from the post-transcriptional to transcriptional level and subsequent spreading of methylation to the NPTII gene.
- Transgenic plants represent an excellent tool for experimental plant biology and are an important component of modern agriculture. Transgenes can be silenced long after their integration. To study the long-term changes in transgene expression in potato, the activity of two reporter genes, encoding GFP and NPTII, was monitored in a set of 17 transgenic lines over 5 years of vegetative propagation in vitro. Complete silencing of the reporter genes was observed in four lines (nearly 25 %), all of which successively silenced the two reporter genes, indicating an interconnection between their silencing. We suggest a hypothetical mechanism involving the successive silencing of the two reporter genes that involves the switch of GFP silencing from the post-transcriptional to transcriptional level and subsequent spreading of methylation to the NPTII gene. (en)
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Title
| - Successive silencing of tandem reporter genes in potato (Solanum tuberosum) over 5 years of vegetative propagation
- Successive silencing of tandem reporter genes in potato (Solanum tuberosum) over 5 years of vegetative propagation (en)
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skos:prefLabel
| - Successive silencing of tandem reporter genes in potato (Solanum tuberosum) over 5 years of vegetative propagation
- Successive silencing of tandem reporter genes in potato (Solanum tuberosum) over 5 years of vegetative propagation (en)
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skos:notation
| - RIV/00216208:11310/10:10082159!RIV11-MSM-11310___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(LC06004), P(LC06034), Z(MSM0021620858)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11310/10:10082159
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - GFP; potato (Solanum tuberosum); transgene silencing (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Fischer, Lukáš
- Opatrný, Zdeněk
- Nocarová, Eva
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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is http://linked.open...avai/riv/vysledek
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