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  • Vliv rozdílných spektrálních intervalů excitace a detekce fluorescence chlorofylu (Chl) a při pokojové teplotě na stanovení využití excitační energie v PS2 byl studován na divokém typu ječmene a jeho mutantu chlorina f2 pěstovaných na nízké a vysoké ozářenosti. Pomocí PAM 101 fluorimetru probíhalo měření ve třech režimech: (1) excitace při 649 nm a detekce v daleké červené oblasti spektra nad 710 nm, (2) excitace při 461 nm a detekce nad 710 nm a (3) excitace v modré oblasti a detekce v červené oblasti (660-710 nm). Nefotochemické zhášení maximální (NPQ) a minimální (SV0) fluorescence byly výrazně vyšší pro modrou excitaci ve srovnání s červenou excitací. Domníváme se, že toto vyplývá z vyšší (cs)
  • The effects of different spectral region of excitation and detection of chlorophyll (Chl) a fluorescence at room temperature on the estimation of excitation energy utilization within PS 2 were studied in wild-type barley and its mutant chlorina f2 grown under low and high irradiances. Three measuring regimes were applied using PAM 101 fluorometer: (1) excitation at 649 nm and detection in the far-red region beyond 710 nm, (2) excitation at 461 nm and detection beyond 710 nm, and (3) excitation in the blue region and detection in the red region (660?710 nm). Non-photochemical quenching of maximal (NPQ) and minimal fluorescence (SV0) were significantly higher for blue excitation as compared to red excitation. We suggest that this results from higher non-radiative dissipation of absorbed excitation energy within LHC2 due to its preferential excitation by blue radiation and from the lower contribution of PS1 emission to the detected fluorescence in the case of blue excitation.
  • The effects of different spectral region of excitation and detection of chlorophyll (Chl) a fluorescence at room temperature on the estimation of excitation energy utilization within PS 2 were studied in wild-type barley and its mutant chlorina f2 grown under low and high irradiances. Three measuring regimes were applied using PAM 101 fluorometer: (1) excitation at 649 nm and detection in the far-red region beyond 710 nm, (2) excitation at 461 nm and detection beyond 710 nm, and (3) excitation in the blue region and detection in the red region (660?710 nm). Non-photochemical quenching of maximal (NPQ) and minimal fluorescence (SV0) were significantly higher for blue excitation as compared to red excitation. We suggest that this results from higher non-radiative dissipation of absorbed excitation energy within LHC2 due to its preferential excitation by blue radiation and from the lower contribution of PS1 emission to the detected fluorescence in the case of blue excitation. (en)
Title
  • Effects of different excitation and detection spectral regions on room temperature chlorophyll a fluorescence parameters
  • Effects of different excitation and detection spectral regions on room temperature chlorophyll a fluorescence parameters (en)
  • Vliv rozdílných spektrálních intervalů excitace a detekce na parametry fluorescence chlorofylu a při pokojové teplotě (cs)
skos:prefLabel
  • Effects of different excitation and detection spectral regions on room temperature chlorophyll a fluorescence parameters
  • Effects of different excitation and detection spectral regions on room temperature chlorophyll a fluorescence parameters (en)
  • Vliv rozdílných spektrálních intervalů excitace a detekce na parametry fluorescence chlorofylu a při pokojové teplotě (cs)
skos:notation
  • RIV/61988987:17310/05:00000005!RIV07-MSM-17310___
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  • 411
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  • P(LN00A141)
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  • 519579
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  • RIV/61988987:17310/05:00000005
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  • Hordeum; non-photochemical quenching; non-radiative dissipation; PAM fluorometer; photosystems 1 and 2 (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [2ED5DEF0A535]
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  • Photosynthetica
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http://linked.open...v/svazekPeriodika
  • 43
http://linked.open...iv/tvurceVysledku
  • Špunda, Vladimír
  • Štroch, Michal
  • Navrátil, Martin
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  • 0300-3604
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  • 17310
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