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  • The flavoenzyme cytokinin dehydrogenace (CKX) catalyzes an irreversible deactivation of plant hormones cytokinins through oxidative cleavage of the cytokinin side chain to Šeld adenine or adenosine and an aldehyde. In the catalytic cycle of CKX, the cytokinin-reduced flavin cofactor is reoxidized by a suitable electron acceptor. We have recently demonstrated that the oxidation products of natural hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) function as effective electron acceptors of apoplastic CKX from maize. The stable oxidation product of DIMBOA reacting with peroxidase or laccase was identified as 4-nitrosoresorcinol 1-monomethyl ether (coniferron), which, however, is only a weak electron acceptor of CKX. Further analyses suggested formativ of transient free radicals that were estimated to reoxidize the cytokininreduced flavin cofactor of CKX with the rates comparable to those of flavin reduction.
  • The flavoenzyme cytokinin dehydrogenace (CKX) catalyzes an irreversible deactivation of plant hormones cytokinins through oxidative cleavage of the cytokinin side chain to Šeld adenine or adenosine and an aldehyde. In the catalytic cycle of CKX, the cytokinin-reduced flavin cofactor is reoxidized by a suitable electron acceptor. We have recently demonstrated that the oxidation products of natural hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) function as effective electron acceptors of apoplastic CKX from maize. The stable oxidation product of DIMBOA reacting with peroxidase or laccase was identified as 4-nitrosoresorcinol 1-monomethyl ether (coniferron), which, however, is only a weak electron acceptor of CKX. Further analyses suggested formativ of transient free radicals that were estimated to reoxidize the cytokininreduced flavin cofactor of CKX with the rates comparable to those of flavin reduction. (en)
Title
  • Function of plant defense secondary metabolite in cytokinin degradation
  • Function of plant defense secondary metabolite in cytokinin degradation (en)
skos:prefLabel
  • Function of plant defense secondary metabolite in cytokinin degradation
  • Function of plant defense secondary metabolite in cytokinin degradation (en)
skos:notation
  • RIV/61389030:_____/10:00359310!RIV11-GA0-61389030
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA301/08/1649), P(GA522/05/0448), Z(AV0Z50380511), Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika
  • 5
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 259930
http://linked.open...ai/riv/idVysledku
  • RIV/61389030:_____/10:00359310
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • cytokinin dehydrogenace; 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one; phenoxyl radiál (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [7B21925A42FA]
http://linked.open...i/riv/nazevZdroje
  • Plant Signalling & Behavior
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 5
http://linked.open...iv/tvurceVysledku
  • Frébortová, Jitka
http://linked.open...n/vavai/riv/zamer
issn
  • 1559-2316
number of pages
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