About: Regulation of phosphoenolpyruvate carboxylase in PVYNTN-infected tobacco plants

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The effect of viral infection on regulation of phosphoenolpyruvate carboxylase in Nicotiana tabacum L. leaves was studied. The PEPC activity was 3 times higher in infected plant leaves as compared to healthy plants. The reason of an increase of PEPC activity in consequence of PVYNTN infection was studied. The amount of PEPC determined by Western blot analysis or qRT PCR of PEPC mRNA did not differ in the control and PVYNTN infected plants. Changes in post-translational modification of PEPC by phosphorylation were evaluated comparing activity of the native and the dephosphorylated enzyme. The infected plants were characterized by a higher decrease of the enzyme activity after its dephosphorylation. Immunochemical detection of phosphoproteins by Western blot analysis showed more intensive band corresponding to PEPC from the infected material. This strengthens the hypothesis of an infection-related phosphorylation, which could be part of the plants response to pathogen attack. The effect of viral infection on regulation of phosphoenolpyruvate carboxylase in Nicotiana tabacum L. leaves was studied. The PEPC activity was 3 times higher in infected plant leaves as compared to healthy plants. The reason of an increase of PEPC activity in consequence of PVYNTN infection was studied. The amount of PEPC determined by Western blot analysis or qRT PCR of PEPC mRNA did not differ in the control and PVYNTN infected plants. Changes in post-translational modification of PEPC by phosphorylation were evaluated comparing activity of the native and the dephosphorylated enzyme. The infected plants were characterized by a higher decrease of the enzyme activity after its dephosphorylation. Immunochemical detection of phosphoproteins by Western blot analysis showed more intensive band corresponding to PEPC from the infected material. This strengthens the hypothesis of an infection-related phosphorylation, which could be part of the plants response to pathogen attack. (en)
Title	Regulation of phosphoenolpyruvate carboxylase in PVYNTN-infected tobacco plants Regulation of phosphoenolpyruvate carboxylase in PVYNTN-infected tobacco plants (en)
skos:prefLabel	Regulation of phosphoenolpyruvate carboxylase in PVYNTN-infected tobacco plants Regulation of phosphoenolpyruvate carboxylase in PVYNTN-infected tobacco plants (en)
skos:notation	RIV/61389030:_____/09:00328462!RIV10-MSM-61389030
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(1M0505), P(GA206/03/0310), S, Z(AV0Z50380511), Z(MSM0021620808)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Štorchová, Helena Čeřovská, Noemi Müller, Karel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	338692
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/09:00328462
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	biotic stress; Nicotiana tabacum; phosphoenolpyruvate carboxylase (PEPC) (en)
http://linked.open.../riv/klicoveSlovo	biotic stress Nicotiana tabacum phosphoenolpyruvate carboxylase (PEPC)
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[DAC7FC88EE09]
http://linked.open...i/riv/nazevZdroje	Biological Chemistry
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of targeted therapeutic drugs Biotic stress caused by viral infection in transgenic Pssu-ipt tobacco
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	390
http://linked.open...iv/tvurceVysledku	Müller, Karel Doubnerová, V. Ryšlavá, H. Čeřovská, Noemi Synková, Helena
http://linked.open...ain/vavai/riv/wos	000264921500005
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy Molekulárně biologické, genetické a epigenetické aspekty vzniku a rozvoje modelových tumorů dospělého věku. Význam pro epidemiologii, časnou diagnostiku a léčbu
issn	1431-6730
number of pages	7 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Regulation of phosphoenolpyruvate carboxylase in PVYNTN-infected tobacco plants Regulation of phosphoenolpyruvate carboxylase in PVYNTN-infected tobacco plants Regulation of phosphoenolpyruvate carboxylase in PVYNTN-infected tobacco plants Regulation of phosphoenolpyruvate carboxylase in PVYNTN-infected tobacco plants

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