About: The bacterial artificial chromosome (BAC) library of the narrow-leafed lupin (Lupinus angustifolius L.)     Goto   Sponge   Distinct   Permalink

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Description
  • The narrow-leafed lupin possesses valuable traits for environment-friendly agriculture and for the production of unconventional agricultural products. Despite various genetic and environmental studies, the breeding of improved cultivars has been slow due to the limited knowledge of its genomic structure. Further advances in genomic require, among other things, the availability of a genomic DNA library with large inserts. We report here on the construction of the first DNA library cloned in a BAC (bacterial artificial chromosome) vector from diploid Lupinus angustifolius L. cv. Sonet. The high molecular weight DNA used for its preparation was isolated from interphase nuclei that were purified by flow cytometry. The library comprises 55,296 clones and is ordered in 144-384-well microtitre plates. With an average insert size of 100 kb, the library represents six haploid genome equivalents. Thanks to the purification of the nuclei by flow cytometry, contamination with chloroplast DNA and mitochondrial ...
  • The narrow-leafed lupin possesses valuable traits for environment-friendly agriculture and for the production of unconventional agricultural products. Despite various genetic and environmental studies, the breeding of improved cultivars has been slow due to the limited knowledge of its genomic structure. Further advances in genomic require, among other things, the availability of a genomic DNA library with large inserts. We report here on the construction of the first DNA library cloned in a BAC (bacterial artificial chromosome) vector from diploid Lupinus angustifolius L. cv. Sonet. The high molecular weight DNA used for its preparation was isolated from interphase nuclei that were purified by flow cytometry. The library comprises 55,296 clones and is ordered in 144-384-well microtitre plates. With an average insert size of 100 kb, the library represents six haploid genome equivalents. Thanks to the purification of the nuclei by flow cytometry, contamination with chloroplast DNA and mitochondrial ... (en)
  • Úzkolisté lupiny mají cenné vlastnosti využitelné v ekologickém zemědělství a pro produkci netradičních zemědělských produktů. Šlechtění vedoucí ke zlepšení kvality existujících odrůd je pomalé díky omezeným znalostem struktury genomu. Další pokrok mimo jiné vyžaduje dostupnost genomické knihovny DNA s velkými inzerty. V tomto příspěvku referujeme o konstrukci první knihovny DNA klonované ve vektoru BAC u diploidního odrůdy Lupinus angustifolius L. cv. Sonet. Vysokomolekulární DNA použitá pro její přípravu byla izolována z interfázních jader, které byly tříděny průtokovým cytometrem. Knihovna zahrnuje 55,296 klonů a je uložena v 144 mikrotitračních destičkách. S průměrnou velikostí inzertů 100 kb, představuje knihovna šest haploidních genomových ekvivalentů. Díky purifikaci jader průtokovou cytometrií byla kontaminace chloroplastovou a mitochondriální DNA zanedbatelná. Dostupnost BAC knihovny umožní vývoj fyzických kontigových map a poziční klonování genů, stejně jako analýzu struktury a evoluce ro... (cs)
Title
  • The bacterial artificial chromosome (BAC) library of the narrow-leafed lupin (Lupinus angustifolius L.)
  • Knihovna klonovaná v umělém bakteriálním chromozómu (BAC) pro lupinu úzkolistou (Lupinus angustifolius L.) (cs)
  • The bacterial artificial chromosome (BAC) library of the narrow-leafed lupin (Lupinus angustifolius L.) (en)
skos:prefLabel
  • The bacterial artificial chromosome (BAC) library of the narrow-leafed lupin (Lupinus angustifolius L.)
  • Knihovna klonovaná v umělém bakteriálním chromozómu (BAC) pro lupinu úzkolistou (Lupinus angustifolius L.) (cs)
  • The bacterial artificial chromosome (BAC) library of the narrow-leafed lupin (Lupinus angustifolius L.) (en)
skos:notation
  • RIV/61389030:_____/06:00082151!RIV07-AV0-61389030
http://linked.open.../vavai/riv/strany
  • 396;407
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(LC06004), S, Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika
  • -
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 466525
http://linked.open...ai/riv/idVysledku
  • RIV/61389030:_____/06:00082151
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • BAC; genomic DNA library; Lupinus angustifolius L. (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • PL - Polská republika
http://linked.open...ontrolniKodProRIV
  • [D418ACDB4363]
http://linked.open...i/riv/nazevZdroje
  • Cellular & Molecular Biology Letters
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 11
http://linked.open...iv/tvurceVysledku
  • Doležel, Jaroslav
  • Šafář, Jan
  • Janda, Jaroslav
  • Naganowska, B.
  • Kasprzak, A.
  • Wolko, B.
http://linked.open...n/vavai/riv/zamer
issn
  • 1425-8153
number of pages
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