About: Sequence analysis of porothramycin biosynthetic gene cluster

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The biosynthetic gene cluster of porothramycin, a sequence-selective DNA alkylating compound, was identified in the genome of producing strain Streptomyces albus subsp. albus (ATCC 39897) and sequentially characterized. A 39.7 kb long DNA region contains 27 putative genes, 18 of them revealing high similarity with homologous genes from biosynthetic gene cluster of closely related pyrrolobenzodiazepine (PBD) compound anthramycin. However, considering the structures of both compounds, the number of differences in the gene composition of compared biosynthetic gene clusters was unexpectedly high, indicating participation of alternative enzymes in biosynthesis of both porothramycin precursors, anthranilate, and branched L-proline derivative. Based on the sequence analysis of putative NRPS modules Por20 and Por21, we suppose that in porothramycin biosynthesis, the methylation of anthranilate unit occurs prior to the condensation reaction, while modifications of branched proline derivative, oxidation, and dimethylation of the side chain occur on already condensed PBD core. The biosynthetic gene cluster of porothramycin, a sequence-selective DNA alkylating compound, was identified in the genome of producing strain Streptomyces albus subsp. albus (ATCC 39897) and sequentially characterized. A 39.7 kb long DNA region contains 27 putative genes, 18 of them revealing high similarity with homologous genes from biosynthetic gene cluster of closely related pyrrolobenzodiazepine (PBD) compound anthramycin. However, considering the structures of both compounds, the number of differences in the gene composition of compared biosynthetic gene clusters was unexpectedly high, indicating participation of alternative enzymes in biosynthesis of both porothramycin precursors, anthranilate, and branched L-proline derivative. Based on the sequence analysis of putative NRPS modules Por20 and Por21, we suppose that in porothramycin biosynthesis, the methylation of anthranilate unit occurs prior to the condensation reaction, while modifications of branched proline derivative, oxidation, and dimethylation of the side chain occur on already condensed PBD core. (en)
Title	Sequence analysis of porothramycin biosynthetic gene cluster Sequence analysis of porothramycin biosynthetic gene cluster (en)
skos:prefLabel	Sequence analysis of porothramycin biosynthetic gene cluster Sequence analysis of porothramycin biosynthetic gene cluster (en)
skos:notation	RIV/61388971:_____/14:00435283!RIV15-AV0-61388971
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(ED1.1.00/02.0109), P(EE2.3.20.0055), P(EE2.3.30.0003)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Kettnerová, Eliška Gažák, Radek Janata, Jiří Jelínková, Markéta Ulanová, Dana Najmanová, Lucie Koběrská, Markéta Kameník, Zdeněk Radojevič, Bojana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	44531
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/14:00435283
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	BIOLOGICAL-ACTIVITY; ANTHRAMYCIN; SPECIFICITY (en)
http://linked.open.../riv/klicoveSlovo	ANTHRAMYCIN BIOLOGICAL-ACTIVITY SPECIFICITY
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[8AC9D7F7EFC7]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	9 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University Establishment of teams for The center of microbiology and immunology IMPULS, Inovation in Microbiology - Postdoctoral training and laboratory center
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	59
http://linked.open...iv/tvurceVysledku	Gažák, Radek Kameník, Zdeněk Jelínková, Markéta Janata, Jiří Koběrská, Markéta Najmanová, Lucie Ulanová, Dana Kettnerová, Eliška Radojevič, Bojana
http://linked.open...ain/vavai/riv/wos	000343812600013
issn	0015-5632
number of pages	10 (xsd:int)

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