About: Analysis of soil fungal communities by amplicon pyrosequencing: current approaches to data analysis and the introduction of the pipeline SEED

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Fungi are important in soils as both decomposers and plant symbionts, and an understanding of the composition of their complex communities is thus indispensable to answer a variety of ecological questions. 454 Pyrosequencing is currently the method of choice for the in-depth analysis of fungal communities. However, the interpretation of its results is complicated by differences in data analysis approaches that make inter-study comparisons difficult. The pyrosequencing studies published so far have also used variable molecular targets in fungal rDNA. Although the ITS region and, in particular, ITS1 appear to be the most frequent sequencing targets, the use of various primers with different coverages of fungal groups remains a serious problem. Sequence length limits also vary widely across studies, and in many studies, length differences may negatively affect sequence similarity clustering or identification Fungi are important in soils as both decomposers and plant symbionts, and an understanding of the composition of their complex communities is thus indispensable to answer a variety of ecological questions. 454 Pyrosequencing is currently the method of choice for the in-depth analysis of fungal communities. However, the interpretation of its results is complicated by differences in data analysis approaches that make inter-study comparisons difficult. The pyrosequencing studies published so far have also used variable molecular targets in fungal rDNA. Although the ITS region and, in particular, ITS1 appear to be the most frequent sequencing targets, the use of various primers with different coverages of fungal groups remains a serious problem. Sequence length limits also vary widely across studies, and in many studies, length differences may negatively affect sequence similarity clustering or identification (en)
Title	Analysis of soil fungal communities by amplicon pyrosequencing: current approaches to data analysis and the introduction of the pipeline SEED Analysis of soil fungal communities by amplicon pyrosequencing: current approaches to data analysis and the introduction of the pipeline SEED (en)
skos:prefLabel	Analysis of soil fungal communities by amplicon pyrosequencing: current approaches to data analysis and the introduction of the pipeline SEED Analysis of soil fungal communities by amplicon pyrosequencing: current approaches to data analysis and the introduction of the pipeline SEED (en)
skos:notation	RIV/61388971:_____/13:00423803!RIV14-MSM-61388971
http://linked.open...avai/predkladatel	Mikrobiologický ústav AV ČR, v.v.i.
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GAP504/12/0709), P(LD12048), P(LD12050)
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Větrovský, Tomáš Baldrian, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	60639
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/13:00423803
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Fungal community; Internal transcribed spacer; Pyrosequencing pipeline (en)
http://linked.open.../riv/klicoveSlovo	Fungal community Pyrosequencing pipeline Internal transcribed spacer
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[84772FE6A898]
http://linked.open...i/riv/nazevZdroje	Biology and Fertility of Soils
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Basidiomycetous fungi: exploration and preservation of biodiversity and involvement in environmental decomposition processes The use of metagenomics and metatranscriptomics for identification of active microorganisms and microbial processes Analysis of environmental metagenomes using next-generation-sequencing and development of data analysis procedures
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	49
http://linked.open...iv/tvurceVysledku	Baldrian, Petr Větrovský, Tomáš
http://linked.open...ain/vavai/riv/wos	000326374900005
issn	0178-2762
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s00374-013-0801-y

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