About: Nuclear Import of Chromatin Remodeler Isw1 Is Mediated by Atypical Bipartite cNLS and Classical Import Pathway

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The protein Isw1 of Saccharomyces cerevisiae is an imitation-switch chromatin-remodeling factor. We studied the mechanisms of its nuclear import and found that the nuclear localization signal (NLS) mediating the transport of Isw1 into the nucleus is located at the end of the C-terminus of the protein (aa10791105). We show that it is an atypical bipartite signal with an unconventional linker of 19 aa (KRIR X19 KKAK) and the only nuclear targeting signal within the Isw1 molecule. The efficiency of Isw1 nuclear import was found to be modulated by changes to the amino acid composition in the vicinity of the KRIR motif, but not by the linker length. Live-cell imaging of various karyopherin mutants and in vitro binding assays of Isw1NLS to importin-a revealed that the nuclear translocation of Isw1 is mediated by the classical import pathway The protein Isw1 of Saccharomyces cerevisiae is an imitation-switch chromatin-remodeling factor. We studied the mechanisms of its nuclear import and found that the nuclear localization signal (NLS) mediating the transport of Isw1 into the nucleus is located at the end of the C-terminus of the protein (aa10791105). We show that it is an atypical bipartite signal with an unconventional linker of 19 aa (KRIR X19 KKAK) and the only nuclear targeting signal within the Isw1 molecule. The efficiency of Isw1 nuclear import was found to be modulated by changes to the amino acid composition in the vicinity of the KRIR motif, but not by the linker length. Live-cell imaging of various karyopherin mutants and in vitro binding assays of Isw1NLS to importin-a revealed that the nuclear translocation of Isw1 is mediated by the classical import pathway (en)
Title	Nuclear Import of Chromatin Remodeler Isw1 Is Mediated by Atypical Bipartite cNLS and Classical Import Pathway Nuclear Import of Chromatin Remodeler Isw1 Is Mediated by Atypical Bipartite cNLS and Classical Import Pathway (en)
skos:prefLabel	Nuclear Import of Chromatin Remodeler Isw1 Is Mediated by Atypical Bipartite cNLS and Classical Import Pathway Nuclear Import of Chromatin Remodeler Isw1 Is Mediated by Atypical Bipartite cNLS and Classical Import Pathway (en)
skos:notation	RIV/61388971:_____/13:00392728!RIV14-MSM-61388971
http://linked.open...avai/predkladatel	Mikrobiologický ústav AV ČR, v.v.i.
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(IAA5020409), P(LC545)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Vašicová, Pavla Strádalová, Vendula Halada, Petr Malcová, Ivana Hašek, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	92487
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/13:00392728
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	bipartite NLS; extended linker; importin-a (en)
http://linked.open.../riv/klicoveSlovo	bipartite NLS extended linker importin-a
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[35150CC52EA0]
http://linked.open...i/riv/nazevZdroje	Traffic
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Functional Organisation of the Cell Yeast ISWI proteins and complexes - analysis of their subcellular localization and their role in the response to saline stress
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	14
http://linked.open...iv/tvurceVysledku	Halada, Petr Strádalová, Vendula Hašek, Jiří Malcová-Janatová, Ivana Vašicová, Pavla
http://linked.open...ain/vavai/riv/wos	000313129100005
issn	1398-9219
number of pages	18 (xsd:int)
http://bibframe.org/vocab/doi	10.1111/tra.12025

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