About: Cyanobacterial Small Chlorophyll-binding Protein ScpD (HliB) Is Located on the Periphery of Photosystem II in the Vicinity of PsbH and CP47 Subunits     Goto   Sponge   Distinct   Permalink

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Description
  • Sinice obsahují několik genů, které kódují malé transmembránové proteiny. Tyto proteiny se nazývají SCP nebo HLIP a mají sekvenční podobnost s chlorofyl a/b-vážícími proteiny z vyšších rostlin. Pomocí dvourozměrné elektroforézy izolovaného jsme identifikovali většinu proteinu ScpD asociovaného s komplexem fotosystému II, a to jak s monomerní ale i dimerní formou. Interakce ScpD proteinu s fotosystemem II byla také studována pomocí elektronové mikroskopie se zlatem značenou afinitní značkou (cs)
  • Cyanobacteria contain several genes coding for small one-helix proteins called SCPs or HLIPs with significant sequence similarity to chlorophyll a/b-binding proteins. Using two-dimensional native-SDS electrophoresis of thylakoid membranes or isolated Photosystem II (PSII), we determined that after high-light treatment most of the ScpDHis protein in a cell is associated with PSII. The ScpDHis protein was present in both monomeric and dimeric PSII core complexes and also in the core subcomplex lacking CP43. However, the association with PSII was abolished in the mutant lacking the PSII subunit PsbH. In a PSII mutant lacking cytochrome b559, which does not accumulate PSII, ScpDHis is associated with CP47. The interaction of ScpDHis with PsbH and CP47 was further confirmed by electron microscopy of PSII labeled with Ni-NTA Nanogold. Single particle image analysis identified the location of the labeled ScpDHis at the periphery of the PSII core complex in the vicinity of the PsbH and CP47
  • Cyanobacteria contain several genes coding for small one-helix proteins called SCPs or HLIPs with significant sequence similarity to chlorophyll a/b-binding proteins. Using two-dimensional native-SDS electrophoresis of thylakoid membranes or isolated Photosystem II (PSII), we determined that after high-light treatment most of the ScpDHis protein in a cell is associated with PSII. The ScpDHis protein was present in both monomeric and dimeric PSII core complexes and also in the core subcomplex lacking CP43. However, the association with PSII was abolished in the mutant lacking the PSII subunit PsbH. In a PSII mutant lacking cytochrome b559, which does not accumulate PSII, ScpDHis is associated with CP47. The interaction of ScpDHis with PsbH and CP47 was further confirmed by electron microscopy of PSII labeled with Ni-NTA Nanogold. Single particle image analysis identified the location of the labeled ScpDHis at the periphery of the PSII core complex in the vicinity of the PsbH and CP47 (en)
Title
  • Cyanobacterial Small Chlorophyll-binding Protein ScpD (HliB) Is Located on the Periphery of Photosystem II in the Vicinity of PsbH and CP47 Subunits
  • Lokalizace malého chlorofyl-vážícího proteinu ScpD (HliB) v blízkosti dvou podjednotek PsbH a CP47 u fotosystemu II ze sinic (cs)
  • Cyanobacterial Small Chlorophyll-binding Protein ScpD (HliB) Is Located on the Periphery of Photosystem II in the Vicinity of PsbH and CP47 Subunits (en)
skos:prefLabel
  • Cyanobacterial Small Chlorophyll-binding Protein ScpD (HliB) Is Located on the Periphery of Photosystem II in the Vicinity of PsbH and CP47 Subunits
  • Lokalizace malého chlorofyl-vážícího proteinu ScpD (HliB) v blízkosti dvou podjednotek PsbH a CP47 u fotosystemu II ze sinic (cs)
  • Cyanobacterial Small Chlorophyll-binding Protein ScpD (HliB) Is Located on the Periphery of Photosystem II in the Vicinity of PsbH and CP47 Subunits (en)
skos:notation
  • RIV/61388971:_____/06:00050785!RIV07-AV0-61388971
http://linked.open.../vavai/riv/strany
  • 32705;32713
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(AV0Z50200510), Z(MSM6007665808)
http://linked.open...iv/cisloPeriodika
  • 43
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 470216
http://linked.open...ai/riv/idVysledku
  • RIV/61388971:_____/06:00050785
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • photosystem II; cyanobacteria; electron microscopy (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [603D3AED9888]
http://linked.open...i/riv/nazevZdroje
  • Journal of Biological Chemistry
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 281
http://linked.open...iv/tvurceVysledku
  • Tichý, Martin
  • Komenda, Josef
  • Nebesářová, J.
  • Bumba, Ladislav
  • Vácha, F.
  • Promnares, Kamoltip
http://linked.open...n/vavai/riv/zamer
issn
  • 0021-9258
number of pages
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