About: Gas chromatographic analysis of amino acid enantiomers in Carbetocin peptide hydrolysates after fast derivatization with pentafluoropropyl chloroformate     Goto   Sponge   Distinct   Permalink

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  • A novel sample preparation protocol for gas chromatographic (GC) analysis of amino acid enantiomers in peptides was developed. It comprises traditional acid hydrolysis, a novel treatment of the analytes with a fluoroalkyl chloroformate and GC/FID separation of enantiomers on a chiral capillary column. The major improvements consist in that the derivatization step proceeds in organic- aqueous media within seconds and the amino acid derivatives are volatile enough to suit the temperature range of the chiral Chirasil-Val capillary column. The approach was found beneficial for chiral analysis of pharmaceutically important Carbetocin peptide.
  • A novel sample preparation protocol for gas chromatographic (GC) analysis of amino acid enantiomers in peptides was developed. It comprises traditional acid hydrolysis, a novel treatment of the analytes with a fluoroalkyl chloroformate and GC/FID separation of enantiomers on a chiral capillary column. The major improvements consist in that the derivatization step proceeds in organic- aqueous media within seconds and the amino acid derivatives are volatile enough to suit the temperature range of the chiral Chirasil-Val capillary column. The approach was found beneficial for chiral analysis of pharmaceutically important Carbetocin peptide. (en)
  • Byl vyvinut nový postup přípravy vzorku pro analýzu enantiomerů aminokyselin v peptidech plynovou chromatografií. Zahrnuje tradiční kyselou hydrolýzu, originální reakci analytů s fluoralkylchlormravenčany a separaci enantiomerů pomocí GC/FID na chirální kapilární koloně. Hlavní zdokonalení metody spočívá v tom, že derivatizační krok probíhá ve vodně-organickém prostředí během několika vteřin, a deriváty aminokyselin jsou natolik těkavé, že mohou být separovány i v omezeném teplotním rozsahu chirální kolony Chirasil-Val. Tento postup byl vhodný pro chirální analýzu aminokyselin ve farmaceuticky významném peptidu Carbetocinu. (cs)
Title
  • Gas chromatographic analysis of amino acid enantiomers in Carbetocin peptide hydrolysates after fast derivatization with pentafluoropropyl chloroformate
  • Gas chromatographic analysis of amino acid enantiomers in Carbetocin peptide hydrolysates after fast derivatization with pentafluoropropyl chloroformate (en)
  • Analýza enantiomerů aminokyselin v peptidu Carbetocinu plynovou chromatografií po derivatizaci pentafluorpropylchlormravenčanem (cs)
skos:prefLabel
  • Gas chromatographic analysis of amino acid enantiomers in Carbetocin peptide hydrolysates after fast derivatization with pentafluoropropyl chloroformate
  • Gas chromatographic analysis of amino acid enantiomers in Carbetocin peptide hydrolysates after fast derivatization with pentafluoropropyl chloroformate (en)
  • Analýza enantiomerů aminokyselin v peptidu Carbetocinu plynovou chromatografií po derivatizaci pentafluorpropylchlormravenčanem (cs)
skos:notation
  • RIV/60077344:_____/08:00310855!RIV09-GA0-60077344
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA203/04/0192), P(GA303/06/1674), Z(AV0Z50070508)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 368939
http://linked.open...ai/riv/idVysledku
  • RIV/60077344:_____/08:00310855
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • D,L-amino acids; peptides; chloroformates (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [49B54E5C1B31]
http://linked.open...i/riv/nazevZdroje
  • Amino Acids
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 35
http://linked.open...iv/tvurceVysledku
  • Hartvich, Petr
  • Hušek, Petr
  • Šimek, Petr
  • Zahradníčková, Helena
http://linked.open...ain/vavai/riv/wos
  • 000258523700027
http://linked.open...n/vavai/riv/zamer
issn
  • 0939-4451
number of pages
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