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Description
  • This work introduces a new approach connecting vibrational spectroscopy with homology and energetic molecular modeling of proteins. Combination of both methods can compensate their disadvantages and result in realistic three-dimensional protein models. The approach is most powerful for membrane proteins or glycoproteins with high carbohydrate content where X-ray or NMR analysis is not always successful. Nevertheless, it can also serve as a tool of preliminary analysis of any protein with unknown structure. Power of the approach is demonstrated on human alpha(1)-acid glycoprotein. Its predicted structure published in [V. Kopecky Jr. et al., Biochem. Biophys. Res. Commun. 300 (2003), 41-46] is discussed in detail with respect to the approach and its general employment.
  • This work introduces a new approach connecting vibrational spectroscopy with homology and energetic molecular modeling of proteins. Combination of both methods can compensate their disadvantages and result in realistic three-dimensional protein models. The approach is most powerful for membrane proteins or glycoproteins with high carbohydrate content where X-ray or NMR analysis is not always successful. Nevertheless, it can also serve as a tool of preliminary analysis of any protein with unknown structure. Power of the approach is demonstrated on human alpha(1)-acid glycoprotein. Its predicted structure published in [V. Kopecky Jr. et al., Biochem. Biophys. Res. Commun. 300 (2003), 41-46] is discussed in detail with respect to the approach and its general employment. (en)
  • Nové metody pro rychlý odhad struktur proteinu byly vyvíjeny a vysvětleny na příkladu lidského orosomukoidu (cs)
Title
  • Vibrational spectroscopy and computer modeling of proteins: solving structure of alpha(1)-acid glycoprotein
  • Vibrational spectroscopy and computer modeling of proteins: solving structure of alpha(1)-acid glycoprotein (en)
  • Vibrační spektroskopie a počítačové modelování proteinu: řešení struktury alpha-1-kyselého glykoproteinu (cs)
skos:prefLabel
  • Vibrational spectroscopy and computer modeling of proteins: solving structure of alpha(1)-acid glycoprotein
  • Vibrational spectroscopy and computer modeling of proteins: solving structure of alpha(1)-acid glycoprotein (en)
  • Vibrační spektroskopie a počítačové modelování proteinu: řešení struktury alpha-1-kyselého glykoproteinu (cs)
skos:notation
  • RIV/60076658:12640/04:00005714!RIV/2005/GA0/126405/N
http://linked.open.../vavai/riv/strany
  • 323-330
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GP206/03/D082), P(LN00A141)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 592396
http://linked.open...ai/riv/idVysledku
  • RIV/60076658:12640/04:00005714
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Raman spectroscopy;fourier transform infrared spectroscopy;molecular modeling;orosomucoid;binding site;thermal dynamics (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [104EA04F8D6C]
http://linked.open...i/riv/nazevZdroje
  • Spectroscopy - An International Journal
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 18
http://linked.open...iv/tvurceVysledku
  • Hofbauerová, Kateřina
  • Kopecký, Vladimír
  • Baumruk, Vladimír
  • Ettrich, Rudiger Horst
issn
  • 0712-4813
number of pages
http://localhost/t...ganizacniJednotka
  • 12640
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