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  • The emergence and alarming increase of multiresistant strains of Staphylococcus aureus emphasizes the need for new and innovative antimicrobial strategies. Polyvalent bacteriophages from the family Myoviridae, sub-family Spounavirinae of genus Twort-like phages that infect S. aureus have potential for use in phage therapy. In this study we characterized genomic sequences of the polyvalent staphylococcal phage 812 and its spontaneous host-range mutants selected on non-susceptible S. aureus strains. The wild type phage was shown to lyse up to 65%, whereas host-range mutants up to 95% of S. aureus strains under study. Genomic DNAs of five phages were sequenced using 454 Genome Sequencer Junior (Roche). Bacteriophage DNA sequences were assembled by software GS De novo Assembler and GS Reference Mapper (Roche). Genome of phage K was chosen as the reference.
  • The emergence and alarming increase of multiresistant strains of Staphylococcus aureus emphasizes the need for new and innovative antimicrobial strategies. Polyvalent bacteriophages from the family Myoviridae, sub-family Spounavirinae of genus Twort-like phages that infect S. aureus have potential for use in phage therapy. In this study we characterized genomic sequences of the polyvalent staphylococcal phage 812 and its spontaneous host-range mutants selected on non-susceptible S. aureus strains. The wild type phage was shown to lyse up to 65%, whereas host-range mutants up to 95% of S. aureus strains under study. Genomic DNAs of five phages were sequenced using 454 Genome Sequencer Junior (Roche). Bacteriophage DNA sequences were assembled by software GS De novo Assembler and GS Reference Mapper (Roche). Genome of phage K was chosen as the reference. (en)
Title
  • Characterization of host-range mutations in genomes of Staphylococcus Twortlikeviruses
  • Characterization of host-range mutations in genomes of Staphylococcus Twortlikeviruses (en)
skos:prefLabel
  • Characterization of host-range mutations in genomes of Staphylococcus Twortlikeviruses
  • Characterization of host-range mutations in genomes of Staphylococcus Twortlikeviruses (en)
skos:notation
  • RIV/00216224:14310/14:00073314!RIV15-MSM-14310___
http://linked.open...avai/riv/aktivita
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  • P(EE2.4.31.0155), P(TA01010405)
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  • S1
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  • 6944
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  • RIV/00216224:14310/14:00073314
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  • bacteriophage therapy; Staphylococcus aureus; Twortlikevirus (en)
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  • RU - Ruská federace
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  • [51E4C02E7A7E]
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  • Russian Journal of Infection and Immunity
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  • 4
http://linked.open...iv/tvurceVysledku
  • Benešík, Martin
  • Boštík, Josef
  • Doškař, Jiří
  • Moša, Marek
  • Pantůček, Roman
  • Mašlaňová, Ivana
  • Varga, Marian
issn
  • 2220-7619
number of pages
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  • 14310
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