About: Cathepsin D expression as a regulator of breast cancer cell migration and chemosensitivity

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The lysosomal aspartic protease cathepsin D (cath-D) is often over-expressed in breast cancer and acts as a mitogen on both cancer and stromal cells. The ability of cath-D to stimulate cancer cell migration remains controversial. To determine whether cath-D expression modulates breast cancer cell migration in vitro, we performed a real-time analysis of MDA-MB-231 breast cancer cell migration using novel methodology based on impedance measurement that enables continuous monitoring of the transition of the cells through a microporous membrane [xCELLigence equipped with CIM-plate 16 (Roche)]. We observed that altering cath-D level by either siRNA or cDNA tranfections significantly affected migration of MDA-MB-231 cells. Tumor cells have often impaired classical caspase-dependent apoptosis pathway and may be therefore more sensitive to agents that trigger alternative cell death pathways. Targeting lysosomes represents a method of choice as many human tumors have increased levels of lysosomal proteases. The lysosomal aspartic protease cathepsin D (cath-D) is often over-expressed in breast cancer and acts as a mitogen on both cancer and stromal cells. The ability of cath-D to stimulate cancer cell migration remains controversial. To determine whether cath-D expression modulates breast cancer cell migration in vitro, we performed a real-time analysis of MDA-MB-231 breast cancer cell migration using novel methodology based on impedance measurement that enables continuous monitoring of the transition of the cells through a microporous membrane [xCELLigence equipped with CIM-plate 16 (Roche)]. We observed that altering cath-D level by either siRNA or cDNA tranfections significantly affected migration of MDA-MB-231 cells. Tumor cells have often impaired classical caspase-dependent apoptosis pathway and may be therefore more sensitive to agents that trigger alternative cell death pathways. Targeting lysosomes represents a method of choice as many human tumors have increased levels of lysosomal proteases. (en)
Title	Cathepsin D expression as a regulator of breast cancer cell migration and chemosensitivity Cathepsin D expression as a regulator of breast cancer cell migration and chemosensitivity (en)
skos:prefLabel	Cathepsin D expression as a regulator of breast cancer cell migration and chemosensitivity Cathepsin D expression as a regulator of breast cancer cell migration and chemosensitivity (en)
skos:notation	RIV/00216224:14310/11:00049467!RIV12-GA0-14310___
http://linked.open...avai/predkladatel	Přírodovědecká fakulta
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA301/09/1115), P(GD204/08/H054), P(IAA501630801), Z(MSM0021622415)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Jančeková, Blanka Šmarda, Jan Knopfová, Lucia
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	189299
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/11:00049467
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cathepsin D; cell migration; breast cancer (en)
http://linked.open.../riv/klicoveSlovo	breast cancer cathepsin D cell migration
http://linked.open...ontrolniKodProRIV	[B97B7D39BD9B]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	The role of c-Myb and Cox-2 proteins in colon carcinogenesis Molecular mechanisms of the cell proliferation and differentiation Mechanism of procathepsin D effect on breast cancer cells
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Beneš, Petr Jančeková, Blanka Knopfová, Lucia Šmarda, Jan
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
http://localhost/t...ganizacniJednotka	14310

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