About: The study on the FnrP, NNR and NarR transcription regulator function in bacterium Paracoccus denitrificans at global proteome level

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Paracoccus denitrificans is a non-fermentative, facultatively autotrophic soil bacterium often studied in the field of bioenergetics. In this work, we aimed to analyze the role of three known transcription regulators FnrP, NNR and NarR (with described role as sensors for oxygen and for intermediates of denitrification pathway) in these adaptation processes in P. denitrificans at global proteome level. We analyzed protein composition of four P. denitrificans strains: wild type, FnrP-, NNR- and NarR- mutants grown aerobically, semiaerobically and semiaerobically with nitrate. Total cell lysate proteins were separated using 2-D PAGE, 36 gels in experiment were statistically evaluated and ca. 500 protein spots were analyzed using MALDI-MS/MS and LC-MS/MS. Paracoccus denitrificans is a non-fermentative, facultatively autotrophic soil bacterium often studied in the field of bioenergetics. In this work, we aimed to analyze the role of three known transcription regulators FnrP, NNR and NarR (with described role as sensors for oxygen and for intermediates of denitrification pathway) in these adaptation processes in P. denitrificans at global proteome level. We analyzed protein composition of four P. denitrificans strains: wild type, FnrP-, NNR- and NarR- mutants grown aerobically, semiaerobically and semiaerobically with nitrate. Total cell lysate proteins were separated using 2-D PAGE, 36 gels in experiment were statistically evaluated and ca. 500 protein spots were analyzed using MALDI-MS/MS and LC-MS/MS. (en)
Title	The study on the FnrP, NNR and NarR transcription regulator function in bacterium Paracoccus denitrificans at global proteome level The study on the FnrP, NNR and NarR transcription regulator function in bacterium Paracoccus denitrificans at global proteome level (en)
skos:prefLabel	The study on the FnrP, NNR and NarR transcription regulator function in bacterium Paracoccus denitrificans at global proteome level The study on the FnrP, NNR and NarR transcription regulator function in bacterium Paracoccus denitrificans at global proteome level (en)
skos:notation	RIV/00216224:14310/08:00025023!RIV11-GA0-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GP203/07/P471), Z(MSM0021622413), Z(MSM0021622415)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Zdráhal, Zbyněk Šedo, Ondrej Struhárová, Iva Bouchal, Pavel Kučera, Igor
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	398200
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/08:00025023
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Paracoccus denitrificans; transcription regulation; metabolic adaptation; proteomics (en)
http://linked.open.../riv/klicoveSlovo	metabolic adaptation Paracoccus denitrificans transcription regulation proteomics
http://linked.open...ontrolniKodProRIV	[115461DEAEF9]
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	The study of molecular mechanisms of bacterial adaptation to environmental changes using proteomic techniques
http://linked.open...UplatneniVysledku	2008
http://linked.open...iv/tvurceVysledku	Zdráhal, Zbyněk Šedo, Ondrej Bouchal, Pavel Kučera, Igor Struhárová, Iva Vyhlídalová, Tereza van Spanning, Rob Reijnders, Willem
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím Molecular basis of cell and tissue regulations
http://localhost/t...ganizacniJednotka	14310

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