About: Study of substrate inhibition by electrophoretically mediated microanalysis in partially filled capillary.

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Substrate inhibition is a common phenomenon in enzyme kinetics. We report here for the first time its study by a combination of the electrophoretically mediated microanalysis (EMMA) methodology with a partial filling technique. In this set-up, the part of capillary is filled with the buffer best for the enzymatic reaction whereas, the rest of the capillary is filled with the background electrolyte optimal for separation of substrates and products. In the case of haloalkane dehalogenase, a model enzyme selected for this study, the enzymatic reaction was performed in 20 mM glycine buffer (pH 8.6) whereas 20 mM beta-alanine - hydrochloric acid buffer (pH 3.5) was used as a background electrolyte in combination with direct detection at 200 nm. The whole study was performed on poorly soluble brominated substrate - 1,2-dibromoethane. Substrate inhibition is a common phenomenon in enzyme kinetics. We report here for the first time its study by a combination of the electrophoretically mediated microanalysis (EMMA) methodology with a partial filling technique. In this set-up, the part of capillary is filled with the buffer best for the enzymatic reaction whereas, the rest of the capillary is filled with the background electrolyte optimal for separation of substrates and products. In the case of haloalkane dehalogenase, a model enzyme selected for this study, the enzymatic reaction was performed in 20 mM glycine buffer (pH 8.6) whereas 20 mM beta-alanine - hydrochloric acid buffer (pH 3.5) was used as a background electrolyte in combination with direct detection at 200 nm. The whole study was performed on poorly soluble brominated substrate - 1,2-dibromoethane. (en)
Title	Study of substrate inhibition by electrophoretically mediated microanalysis in partially filled capillary. Study of substrate inhibition by electrophoretically mediated microanalysis in partially filled capillary. (en)
skos:prefLabel	Study of substrate inhibition by electrophoretically mediated microanalysis in partially filled capillary. Study of substrate inhibition by electrophoretically mediated microanalysis in partially filled capillary. (en)
skos:notation	RIV/00216224:14310/07:00020038!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/06/0047), P(LC06023), Z(MSM0021622413)
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Němec, Tomáš Papežová, Kateřina Chaloupková, Radka Glatz, Zdeněk
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	453316
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/07:00020038
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	CE; EMMA substrate; inhibition; haloalkane dehalogenase (en)
http://linked.open.../riv/klicoveSlovo	inhibition EMMA substrate CE haloalkane dehalogenase
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[B515974CE304]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography A
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Integrated bioanalytical technologies for microanalyses and diagnostics with laser induced fluorescence and mass spectrometry coupling. Capillary electrophoresis as a tool for the drug-metabolism studies
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	1150
http://linked.open...iv/tvurceVysledku	Chaloupková, Radka Němec, Tomáš Glatz, Zdeněk Papežová, Kateřina
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím
issn	0021-9673
number of pages	5 (xsd:int)
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Study of substrate inhibition by electrophoretically mediated microanalysis in partially filled capillary. Study of substrate inhibition by electrophoretically mediated microanalysis in partially filled capillary. Study of substrate inhibition by electrophoretically mediated microanalysis in partially filled capillary.

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