About: Determination of isoflavones using high-performance liquid chromatography with electrochemical detection (HPLC- ED)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Stanovení isoflavonů za použití HPLC s elektrochemickou detekcí (cs) A highly sensitive HPLC-ED was developed for the determination of isoflavones. The electrochemical behaviour of daidzein and genistein was studied on a carbon paste electrode by square wave voltammetry. The electrochemical results obtained were used for the development of the HPLC-ED method. The HPLC-ED system used consisted of two solvent delivery pumps and an eight-channel CoulArray electrochemical detector. Isoflavones were separated on an Atlantis dC18 column using a mobile phase consisting of acetonitrile (solvent A) and 0.15 M acetate buffer of pH 5.5 (solvent B) at a flow rate of 0.4 mL/min. The full scan of multi-channel coulometric detection was tested and an optimal potential at 450 mV was chosen for our purposes. The calibration curves were linear (daidzein R2 = 0.9993 and genistein R2 = 0.9987). The relative standard deviation (R.S.D., n = 5) was 2.5 %. The detection limits for daidzein and genistein were 480 and 394 pg/mL (1.8 and 1.5 nM), respectively. Isoflavones extracted from soybean A highly sensitive HPLC-ED was developed for the determination of isoflavones. The electrochemical behaviour of daidzein and genistein was studied on a carbon paste electrode by square wave voltammetry. The electrochemical results obtained were used for the development of the HPLC-ED method. The HPLC-ED system used consisted of two solvent delivery pumps and an eight-channel CoulArray electrochemical detector. Isoflavones were separated on an Atlantis dC18 column using a mobile phase consisting of acetonitrile (solvent A) and 0.15 M acetate buffer of pH 5.5 (solvent B) at a flow rate of 0.4 mL/min. The full scan of multi-channel coulometric detection was tested and an optimal potential at 450 mV was chosen for our purposes. The calibration curves were linear (daidzein R2 = 0.9993 and genistein R2 = 0.9987). The relative standard deviation (R.S.D., n = 5) was 2.5 %. The detection limits for daidzein and genistein were 480 and 394 pg/mL (1.8 and 1.5 nM), respectively. Isoflavones extracted from soybean (en)
Title	Stanovení isoflavonů za použití HPLC s elektrochemickou detekcí (cs) Determination of isoflavones using high-performance liquid chromatography with electrochemical detection (HPLC- ED) Determination of isoflavones using high-performance liquid chromatography with electrochemical detection (HPLC- ED) (en)
skos:prefLabel	Stanovení isoflavonů za použití HPLC s elektrochemickou detekcí (cs) Determination of isoflavones using high-performance liquid chromatography with electrochemical detection (HPLC- ED) Determination of isoflavones using high-performance liquid chromatography with electrochemical detection (HPLC- ED) (en)
skos:notation	RIV/00216224:14310/04:00011236!RIV08-AV0-14310___
http://linked.open.../vavai/riv/strany	433 - 433
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA203/02/0422), P(IAA1163201)
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Kubáň, Vlastimil Trnková, Libuše Zehnálek, Josef Kizek, René Potěšil, David
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	560082
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/04:00011236
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	HPLC-ED; square wave voltammetry; isoflavones; daidzein; genistein; soybean products (en)
http://linked.open.../riv/klicoveSlovo	soybean products genistein square wave voltammetry HPLC-ED isoflavones daidzein
http://linked.open...ontrolniKodProRIV	[C4A5FA69E1C6]
http://linked.open...v/mistoKonaniAkce	Thessaloniki, Greece
http://linked.open...i/riv/mistoVydani	Thessaloniki, Greece
http://linked.open...i/riv/nazevZdroje	Book of Abstracts - 55th Annual Meeting of the ISE
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.
http://linked.open...UplatneniVysledku	2004
http://linked.open...iv/tvurceVysledku	Kizek, René Kubáň, Vlastimil Trnková, Libuše Zehnálek, Josef Klejdus, Bořivoj Petrlová, Jitka Mikelová, Radka Potěšil, David
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2004-09-19 (xsd:date)
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	International Society of Electrochemistry
http://localhost/t...ganizacniJednotka	14310

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