About: Determination of isoflavones in soybean food and human urine using liquid chromatography with electrochemical detection

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A highly sensitive high-performance liquid chromatographic method with electrochemical detection (HPLC-ED) was developed for the determination of isoflavones. Electrochemical behaviour of daidzein and genistein was studied on carbon paste electrode (CPE) by adsorptive transfer stripping square wave voltammetry. The obtained electrochemical results were used for the development of HPLC-ED method. Furthermore, isoflavones were separated on an Atlantis dC18 column using a mobile phase consisting of acetonitrile (solvent A) and 0.15M acetate buffer of pH 5.5 (solvent B) at a flow rate 0.4 mL/min. A linear gradient profile (solvent B) was at 02 min 87%; 22 min 60%; 27 min 50%; 31 min 45%; 47 min 87%. Full scan of multi-channel coulometric detection was tested and optimal potential at 450mV was chosen for our purposes. Calibration curves were linear (daidzein R2 = 0.9993 and genistein R2 = 0.9987). The detection limit for daidzein/genistein was 480/394 pg/mL (1.8/1.5 nM) and per column 2.4/1.9 pg. Isoflavon A highly sensitive high-performance liquid chromatographic method with electrochemical detection (HPLC-ED) was developed for the determination of isoflavones. Electrochemical behaviour of daidzein and genistein was studied on carbon paste electrode (CPE) by adsorptive transfer stripping square wave voltammetry. The obtained electrochemical results were used for the development of HPLC-ED method. Furthermore, isoflavones were separated on an Atlantis dC18 column using a mobile phase consisting of acetonitrile (solvent A) and 0.15M acetate buffer of pH 5.5 (solvent B) at a flow rate 0.4 mL/min. A linear gradient profile (solvent B) was at 02 min 87%; 22 min 60%; 27 min 50%; 31 min 45%; 47 min 87%. Full scan of multi-channel coulometric detection was tested and optimal potential at 450mV was chosen for our purposes. Calibration curves were linear (daidzein R2 = 0.9993 and genistein R2 = 0.9987). The detection limit for daidzein/genistein was 480/394 pg/mL (1.8/1.5 nM) and per column 2.4/1.9 pg. Isoflavon (en) Stanovení isoflavon v sojových bobech a v lidské moi za použití HPLC s elektrochemickou detekcí (cs)
Title	Determination of isoflavones in soybean food and human urine using liquid chromatography with electrochemical detection Determination of isoflavones in soybean food and human urine using liquid chromatography with electrochemical detection (en) Stanovení isoflavon v sojových bobech a v lidské moi za použití HPLC s elektrochemickou detekcí (cs)
skos:prefLabel	Determination of isoflavones in soybean food and human urine using liquid chromatography with electrochemical detection Determination of isoflavones in soybean food and human urine using liquid chromatography with electrochemical detection (en) Stanovení isoflavon v sojových bobech a v lidské moi za použití HPLC s elektrochemickou detekcí (cs)
skos:notation	RIV/00216224:14310/04:00011226!RIV08-AV0-14310___
http://linked.open.../vavai/riv/strany	101 - 111
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/02/0422), P(IAA1163201), Z(MSM 432100001)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Kubáň, Vlastimil Trnková, Libuše Adam, Vojtěch Zehnálek, Josef Kizek, René
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	560081
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/04:00011226
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Isoflavones; HPLC; electrochemical detection (en)
http://linked.open.../riv/klicoveSlovo	Isoflavones electrochemical detection HPLC
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[7B1F48453785]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography B
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	806
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Kizek, René Kubáň, Vlastimil Trnková, Libuše Zehnálek, Josef Vacek, Jan Klejdus, Borivoj
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20432100001
issn	1570-0232
number of pages	11 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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