About: Complex Study of Haloalkane Dehalogenase by Means of Electrophoretlcally Mediated Microanalysis

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Halogenated aliphatic hydrocarbons constitute one of the largest groups of environmental pollutants. Haloalkane dehalogenases are a group of enzymes involved in the biodegradation of these compounds by catalysing cleavage of the carbon-halogen bond. The study of the biochemistry of dehalogenation processes may help to understand and evaluate the potential for their degradation in nature. This work aims to develop Electrophoretically Mediated MicroAnalysis (EMMA)-based assays for the complex study of haloalkane dehalogenase. EMMA utilizes the different electrophoretic mobilities of enzyme, substrate and product to initiate reaction inside the capillary and to separate components from each other for final in-capillary quantitation. The Michaelis constants for different substrates, the inhibition constant for inhibitor 1,2-dichloroethane, substrate inhibition of 1,2-dibromoethane and the effect of temperature on enzymatic reaction of haloalkane dehalogenase from Sphingomonas paucimobilis UT26 were evalua Halogenated aliphatic hydrocarbons constitute one of the largest groups of environmental pollutants. Haloalkane dehalogenases are a group of enzymes involved in the biodegradation of these compounds by catalysing cleavage of the carbon-halogen bond. The study of the biochemistry of dehalogenation processes may help to understand and evaluate the potential for their degradation in nature. This work aims to develop Electrophoretically Mediated MicroAnalysis (EMMA)-based assays for the complex study of haloalkane dehalogenase. EMMA utilizes the different electrophoretic mobilities of enzyme, substrate and product to initiate reaction inside the capillary and to separate components from each other for final in-capillary quantitation. The Michaelis constants for different substrates, the inhibition constant for inhibitor 1,2-dichloroethane, substrate inhibition of 1,2-dibromoethane and the effect of temperature on enzymatic reaction of haloalkane dehalogenase from Sphingomonas paucimobilis UT26 were evalua (en) Jedná se o komplexní studii enzymu haloalkandehalogeansy metodou EMMA. (cs)
Title	Komplexní studie enzymu haloalkandehalogeansy metodou EMMA (cs) Complex Study of Haloalkane Dehalogenase by Means of Electrophoretlcally Mediated Microanalysis Complex Study of Haloalkane Dehalogenase by Means of Electrophoretlcally Mediated Microanalysis (en)
skos:prefLabel	Komplexní studie enzymu haloalkandehalogeansy metodou EMMA (cs) Complex Study of Haloalkane Dehalogenase by Means of Electrophoretlcally Mediated Microanalysis Complex Study of Haloalkane Dehalogenase by Means of Electrophoretlcally Mediated Microanalysis (en)
skos:notation	RIV/00216224:14310/04:00010517!RIV09-GA0-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/03/1025), P(GA203/03/1125), Z(MSM 143100005)
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Telnarová, Magdalena Vytisková, Soňa Papežová, Kateřina Glatz, Zdeněk
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	558405
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/04:00010517
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	microcystins; capillary electrochromatography (en)
http://linked.open.../riv/klicoveSlovo	capillary electrochromatography microcystins
http://linked.open...ontrolniKodProRIV	[1418D7B1C28C]
http://linked.open...v/mistoKonaniAkce	ím Itálie
http://linked.open...i/riv/mistoVydani	ím
http://linked.open...i/riv/nazevZdroje	Book of abstract 14 th International Symposium on Capillary Electroseparation Techniques ITP 2004
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Use of the real-time PCR for fetal DNA analysis in maternal plasma Application of capillary zone electrophoresis for the study of enzymes
http://linked.open...UplatneniVysledku	2004
http://linked.open...iv/tvurceVysledku	Glatz, Zdeněk Vytisková, Soňa Papežová, Kateřina Telnarová, Magdalena
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2004-09-12 (xsd:date)
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20143100005
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Consiglio Nazionale delle Ricerche
http://localhost/t...ganizacniJednotka	14310

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