About: Cyanide hydratase from Aspergillus niger K10: Overproduction in Escherichia coli, purification, characterization and use in continuous cyanide degradation

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1016/j.procbio.2013.12.008
Description	A cyanide hydratase from Aspergillus niger K10 was expressed in Escherichia coli and purified. Apart from HCN, it transformed some nitriles, preferentially 2-cyanopyridine and fumaronitrile. V-max and K-m for HCN were ca. 6.8mmol min(-1) mg(-1) protein and 109mM, respectively. V-max for fumaronitrite and 2-cyanopyridine was two to three orders of magnitude lower than for HCN (ca. 18.8 and 10.3 mu.,mol min(-1) mg(-1), respectively) but K-m was also lower (ca. 14.7 and 3.7 mM, respectively). Both cyanide hydratase and nitrilase activities were abolished in truncated enzyme variants missing 18-34 C-terminal aa residues. The enzyme exhibited the highest activity at 45 degrees C and pH 8-9; it was unstable at over 35 degrees C and at below pH 5.5. The operational stability of the whole-cell catalyst was examined in continuous stirred membrane reactors with 70-mL working volume. The catalyst exhibited a half-life of 5.6 h at 28 degrees C. A reactor loaded with an excess of the catalyst was used to degrade 25 mM KCN. A conversion rate of over 80% was maintained for 3 days. A cyanide hydratase from Aspergillus niger K10 was expressed in Escherichia coli and purified. Apart from HCN, it transformed some nitriles, preferentially 2-cyanopyridine and fumaronitrile. V-max and K-m for HCN were ca. 6.8mmol min(-1) mg(-1) protein and 109mM, respectively. V-max for fumaronitrite and 2-cyanopyridine was two to three orders of magnitude lower than for HCN (ca. 18.8 and 10.3 mu.,mol min(-1) mg(-1), respectively) but K-m was also lower (ca. 14.7 and 3.7 mM, respectively). Both cyanide hydratase and nitrilase activities were abolished in truncated enzyme variants missing 18-34 C-terminal aa residues. The enzyme exhibited the highest activity at 45 degrees C and pH 8-9; it was unstable at over 35 degrees C and at below pH 5.5. The operational stability of the whole-cell catalyst was examined in continuous stirred membrane reactors with 70-mL working volume. The catalyst exhibited a half-life of 5.6 h at 28 degrees C. A reactor loaded with an excess of the catalyst was used to degrade 25 mM KCN. A conversion rate of over 80% was maintained for 3 days. (en)
Title	Cyanide hydratase from Aspergillus niger K10: Overproduction in Escherichia coli, purification, characterization and use in continuous cyanide degradation Cyanide hydratase from Aspergillus niger K10: Overproduction in Escherichia coli, purification, characterization and use in continuous cyanide degradation (en)
skos:prefLabel	Cyanide hydratase from Aspergillus niger K10: Overproduction in Escherichia coli, purification, characterization and use in continuous cyanide degradation Cyanide hydratase from Aspergillus niger K10: Overproduction in Escherichia coli, purification, characterization and use in continuous cyanide degradation (en)
skos:notation	RIV/00216208:11310/14:10289017!RIV15-MSM-11310___
http://linked.open...avai/riv/aktivita	I P S
http://linked.open...avai/riv/aktivity	I, P(GAP504/11/0394), S
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Veselá, Alícia Barbara Petříčková, Alena
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	9489
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/14:10289017
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Continuous stirred membrane reactor; Aspergillus niger; Nitrilase; Cyanide hydratase (en)
http://linked.open.../riv/klicoveSlovo	Cyanide hydratase Aspergillus niger Nitrilase Continuous stirred membrane reactor
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[42907B827CB9]
http://linked.open...i/riv/nazevZdroje	Process Biochemistry
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Interspecies comparison of nitrilases by using recombinant enzymes obtained by database-mining: their environmental impact and potential application
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	49
http://linked.open...iv/tvurceVysledku	Kaplan, Ondřej Martínková, Ludmila Plíhal, Ondřej Rinágelová, Anna Pasquarelli, Fabrizia Veselá, Alicja Barbara Cantarella, Maria Chmátal, Martin Křenková, Alena
http://linked.open...ain/vavai/riv/wos	000334482300013
issn	1359-5113
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.procbio.2013.12.008
http://localhost/t...ganizacniJednotka	11310

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