About: Ato protein interactions in yeast plasma membrane revealed by fluorescence lifetime imaging (FLIM)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.sciencedirect.com/science/article/pii/S0005273612001538
Description	Each of the three plasma membrane Ato proteins is involved in ammonium signalling and the development of yeast colonies. This suggests that although these proteins are homologous, they do not functionally substitute for each other, but may forma functional complex. Here,we present a detailed combined FRET, FLIM and photobleaching study, which enabled us to detect interactions between Ato proteins found in distinct compartments of yeast cells. We thus show that the proteins Ato1p and Ato2p interact and can form complexes when present in the plasmamembrane. No interaction was detected between Ato1p and Ato3p or Ato2p and Ato3p. In addition, using specially prepared strains, we were able to detect an interaction between molecules of the same Ato protein, namely Ato1p-Ato1p and Ato3p-Ato3p, but not Ato2p-Ato2p. Each of the three plasma membrane Ato proteins is involved in ammonium signalling and the development of yeast colonies. This suggests that although these proteins are homologous, they do not functionally substitute for each other, but may forma functional complex. Here,we present a detailed combined FRET, FLIM and photobleaching study, which enabled us to detect interactions between Ato proteins found in distinct compartments of yeast cells. We thus show that the proteins Ato1p and Ato2p interact and can form complexes when present in the plasmamembrane. No interaction was detected between Ato1p and Ato3p or Ato2p and Ato3p. In addition, using specially prepared strains, we were able to detect an interaction between molecules of the same Ato protein, namely Ato1p-Ato1p and Ato3p-Ato3p, but not Ato2p-Ato2p. (en)
Title	Ato protein interactions in yeast plasma membrane revealed by fluorescence lifetime imaging (FLIM) Ato protein interactions in yeast plasma membrane revealed by fluorescence lifetime imaging (FLIM) (en)
skos:prefLabel	Ato protein interactions in yeast plasma membrane revealed by fluorescence lifetime imaging (FLIM) Ato protein interactions in yeast plasma membrane revealed by fluorescence lifetime imaging (FLIM) (en)
skos:notation	RIV/00216208:11310/12:10120331!RIV13-GA0-11310___
http://linked.open...avai/predkladatel	Přírodovědecká fakulta
http://linked.open...avai/riv/aktivita	I P S Z
http://linked.open...avai/riv/aktivity	I, P(GA204/08/0718), P(LC06063), P(LC531), S, Z(AV0Z40400503), Z(MSM0021620858)
http://linked.open...iv/cisloPeriodika	9
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Holoubek, Aleš Kučerová, Helena Palková, Zdena Strachotová, Dita
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	124018
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/12:10120331
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Donor lifetime; Plasma membrane protein interaction; Homo/hetero di(oligo)mers; FLIM-photobleaching technique; Ammonium exporters Ato1p, Ato2p and Ato3p (en)
http://linked.open.../riv/klicoveSlovo	Ammonium exporters Ato1p Ato2p and Ato3p Donor lifetime FLIM-photobleaching technique Homo/hetero di(oligo)mers Plasma membrane protein interaction
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[7DA53F33013F]
http://linked.open...i/riv/nazevZdroje	Biochimica et Biophysica Acta - Biomembranes
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Flurescence microscopy in biological and medical research Centre on molecular biology and physiology of yeast communities Role of ammonia signalling in yeast colony differentiation, development, ageing and survival: Molecular mechanisms and functions.
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	1818
http://linked.open...iv/tvurceVysledku	Váchová, Libuše Holoubek, Aleš Humpolíčková, Jana Benda, Aleš Kučerová, Helena Palková, Zdena Strachotová, Dita
http://linked.open...ain/vavai/riv/wos	000306882600006
http://linked.open...n/vavai/riv/zamer	Struktura, reaktivita a dynamika molekulárních a biomolekulárních systémů: teorie, experiment, aplikace Signaling and cellular response mechanisms
issn	0005-2736
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.bbamem.2012.05.005
http://localhost/t...ganizacniJednotka	11310
is http://linked.open...avai/riv/vysledek of	Ato protein interactions in yeast plasma membrane revealed by fluorescence lifetime imaging (FLIM)

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