About: Effect of Potato virus Y on the activities of antioxidant and anaplerotic enzymes in Nicotiana tabacum L. transgenic plants transformed with the gene for P3 protein

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Description	The response of transgenic Nicotiana tabacum L. plants carrying the gene for potyviral non-structural P3 protein to Potato virus Y (strain NTN) infection was not significantly different from the wild type. The activities of antioxidant enzymes catalase (CAT), glutathion reductase (GR), ascorbate peroxidase (APOD) and superoxid dismutase (SOD), determined in systemically infected leaves at early stages of the infection did not change except for the transient increase in CAT and GR activities in the wild type infected plants compared to the healthy ones. At the stages, when severe symptoms of the infection developed on the leaves and the virus content reached its maximum, a significant increase in the activities of several enzymes of anaplerotic metabolic pathways, NADP-dependent malic enzyme (NADP-ME), phosphoenolpyruvate carboxylase (PEPC), and pyruvate, orthophosphate dikinase (PPDK) in transgenic plants was found, similarly as in the wild type. The response of transgenic Nicotiana tabacum L. plants carrying the gene for potyviral non-structural P3 protein to Potato virus Y (strain NTN) infection was not significantly different from the wild type. The activities of antioxidant enzymes catalase (CAT), glutathion reductase (GR), ascorbate peroxidase (APOD) and superoxid dismutase (SOD), determined in systemically infected leaves at early stages of the infection did not change except for the transient increase in CAT and GR activities in the wild type infected plants compared to the healthy ones. At the stages, when severe symptoms of the infection developed on the leaves and the virus content reached its maximum, a significant increase in the activities of several enzymes of anaplerotic metabolic pathways, NADP-dependent malic enzyme (NADP-ME), phosphoenolpyruvate carboxylase (PEPC), and pyruvate, orthophosphate dikinase (PPDK) in transgenic plants was found, similarly as in the wild type. (en)
Title	Effect of Potato virus Y on the activities of antioxidant and anaplerotic enzymes in Nicotiana tabacum L. transgenic plants transformed with the gene for P3 protein Effect of Potato virus Y on the activities of antioxidant and anaplerotic enzymes in Nicotiana tabacum L. transgenic plants transformed with the gene for P3 protein (en)
skos:prefLabel	Effect of Potato virus Y on the activities of antioxidant and anaplerotic enzymes in Nicotiana tabacum L. transgenic plants transformed with the gene for P3 protein Effect of Potato virus Y on the activities of antioxidant and anaplerotic enzymes in Nicotiana tabacum L. transgenic plants transformed with the gene for P3 protein (en)
skos:notation	RIV/00216208:11310/07:13683!RIV10-MSM-11310___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(1M0505), S, Z(MSM0021620808)
http://linked.open...iv/cisloPeriodika	3-4
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Doubnerová, Veronika Ryšlavá, Helena Karásková, Martina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	419052
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/07:13683
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	antioxidant enzymes; P3 protein; biotic stress; NADP-malic enzyme; PEPC (en)
http://linked.open.../riv/klicoveSlovo	NADP-malic enzyme antioxidant enzymes PEPC biotic stress P3 protein
http://linked.open...odStatuVydavatele	BG - Bulharská republika
http://linked.open...ontrolniKodProRIV	[0605DAE9A1FB]
http://linked.open...i/riv/nazevZdroje	General and Applied Plant Physiology
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of targeted therapeutic drugs
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	33
http://linked.open...iv/tvurceVysledku	Doubnerová, Veronika Ryšlavá, Helena Čeřovská, Noemi Synková, Helena Šubr, Zdeno Janošková, Martina
http://linked.open...n/vavai/riv/zamer	Molekulárně biologické, genetické a epigenetické aspekty vzniku a rozvoje modelových tumorů dospělého věku. Význam pro epidemiologii, časnou diagnostiku a léčbu
issn	1312-8183
number of pages	19 (xsd:int)
http://localhost/t...ganizacniJednotka	11310
is http://linked.open...avai/riv/vysledek of	Effect of Potato virus Y on the activities of antioxidant and anaplerotic enzymes in Nicotiana tabacum L. transgenic plants transformed with the gene for P3 protein Effect of Potato virus Y on the activities of antioxidant and anaplerotic enzymes in Nicotiana tabacum L. transgenic plants transformed with the gene for P3 protein Effect of Potato virus Y on the activities of antioxidant and anaplerotic enzymes in Nicotiana tabacum L. transgenic plants transformed with the gene for P3 protein

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