About: In situ fluorescence visualization of bromouridine incorporated into newly transcribed nucleolar RNA

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About: In situ fluorescence visualization of bromouridine incorporated into newly transcribed nucleolar RNA Goto Sponge Distinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1078/0065-1281-00535
Description	Bromouridine-triphosphate is commonly used for in situ immunocytochemical labeling of newly synthesized RNA in living cells. While extranucleolar transcripts do not require special conditions for visualization, special treatment prior to fixation (e. g. incubation with alpha-amanitine) is necessary for immunofluorescence detection of bromouridine-labeled nucleolar RNA in previous studies. We show in the present investigation that bromouridine-triphosphate is efficiently used by both extranucleolar and nucleolar RNA polymerases in living cultured cells. The failure to detect incorporated bromouridine within nucleoli is entirely due to improper treatment of cells after bromouridine incorporation. When methanol/acetone fixation is used, fluorescence signals within nucleoli can be routinely found. Bromouridine-triphosphate is commonly used for in situ immunocytochemical labeling of newly synthesized RNA in living cells. While extranucleolar transcripts do not require special conditions for visualization, special treatment prior to fixation (e. g. incubation with alpha-amanitine) is necessary for immunofluorescence detection of bromouridine-labeled nucleolar RNA in previous studies. We show in the present investigation that bromouridine-triphosphate is efficiently used by both extranucleolar and nucleolar RNA polymerases in living cultured cells. The failure to detect incorporated bromouridine within nucleoli is entirely due to improper treatment of cells after bromouridine incorporation. When methanol/acetone fixation is used, fluorescence signals within nucleoli can be routinely found. (en)
Title	In situ fluorescence visualization of bromouridine incorporated into newly transcribed nucleolar RNA In situ fluorescence visualization of bromouridine incorporated into newly transcribed nucleolar RNA (en)
skos:prefLabel	In situ fluorescence visualization of bromouridine incorporated into newly transcribed nucleolar RNA In situ fluorescence visualization of bromouridine incorporated into newly transcribed nucleolar RNA (en)
skos:notation	RIV/00216208:11120/00:00003749!RIV12-MSM-11120___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA302/99/0587), P(GV304/96/K002), P(VS96129), Z(AV0Z5039906)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Kopský, Vojtěch Raška, Ivan Staněk, David Malinský, Jan Koberna, Karel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / 3. lékařská fakulta
http://linked.open...dnocenehoVysledku	713323
http://linked.open...ai/riv/idVysledku	RIV/00216208:11120/00:00003749
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	bromouridine-triphosphate; transcription; RNA polymerase I; immunofluorescence (en)
http://linked.open.../riv/klicoveSlovo	RNA polymerase I transcription immunofluorescence bromouridine-triphosphate
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[A7DCCDF4E62A]
http://linked.open...i/riv/nazevZdroje	Acta Histochemica
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Functional organization of the cell nucleus Topography of nuclear architecture associated with the RNA synthesis,processing and transport. Laboratory of gene expression
http://linked.open...UplatneniVysledku	2000
http://linked.open...v/svazekPeriodika	102
http://linked.open...iv/tvurceVysledku	Raška, Ivan Staněk, David Malínský, Jan Novotná, Jana Koberna, Karel Čtrnáctá, Vlasta Kopský, Vojtěch Cermanová, Štěpánka
http://linked.open...ain/vavai/riv/wos	000085873100002
http://linked.open...n/vavai/riv/zamer	The molecular and cellular basis of selected human diseases, mechanisms of normal and pathological function, principles of diagnosis and therapy
issn	0065-1281
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1078/0065-1281-00535
http://localhost/t...ganizacniJednotka	11120

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