About: NO Formation by Neuronal NO-Synthase can be Controlled by Ultrafast Electron Injection from a Nanotrigger

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Nitric oxide synthases (NOSs) are unique flavohemoproteins with various roles in mammalian physiology. Constitutive NOS catalysis is initiated by fast hydride transfer from NADPH, followed by slower structural rearrangements. We used a photoactive nanotrigger (NT) to study the initial electron transfer to FAD in native neuronal NOS (nNOS) catalysis. Molecular modeling and fluorescence spectroscopy showed that selective NT binding to NADPH sites close to FAD is able to override Phe1395 regulation. Ultrafast injection of electrons into the protein electron pathway by NT photoactivation through the use of a femtosecond laser pulse is thus possible. We show that calmodulin, required for NO synthesis by constitutive NOS, strongly promotes intramolecular electron flow (6.2-fold stimulation) by a mechanism involving proton transfer to the reduced FAD(-) site. Site-directed mutagenesis using the S1176A and S1776T mutants of nNOS supports this hypothesis. Nitric oxide synthases (NOSs) are unique flavohemoproteins with various roles in mammalian physiology. Constitutive NOS catalysis is initiated by fast hydride transfer from NADPH, followed by slower structural rearrangements. We used a photoactive nanotrigger (NT) to study the initial electron transfer to FAD in native neuronal NOS (nNOS) catalysis. Molecular modeling and fluorescence spectroscopy showed that selective NT binding to NADPH sites close to FAD is able to override Phe1395 regulation. Ultrafast injection of electrons into the protein electron pathway by NT photoactivation through the use of a femtosecond laser pulse is thus possible. We show that calmodulin, required for NO synthesis by constitutive NOS, strongly promotes intramolecular electron flow (6.2-fold stimulation) by a mechanism involving proton transfer to the reduced FAD(-) site. Site-directed mutagenesis using the S1176A and S1776T mutants of nNOS supports this hypothesis. (en)
Title	NO Formation by Neuronal NO-Synthase can be Controlled by Ultrafast Electron Injection from a Nanotrigger NO Formation by Neuronal NO-Synthase can be Controlled by Ultrafast Electron Injection from a Nanotrigger (en)
skos:prefLabel	NO Formation by Neuronal NO-Synthase can be Controlled by Ultrafast Electron Injection from a Nanotrigger NO Formation by Neuronal NO-Synthase can be Controlled by Ultrafast Electron Injection from a Nanotrigger (en)
skos:notation	RIV/00216208:11110/09:4029!RIV10-MSM-11110___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(KAN200200651), Z(MSM0021620806)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Martásek, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / 1. lékařská fakulta
http://linked.open...dnocenehoVysledku	329623
http://linked.open...ai/riv/idVysledku	RIV/00216208:11110/09:4029
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	emission spectroscopy; flavins; kinetics; metalloenzymes; molecular modeling; photocatalysis; nitric-oxide synthase; redox properties; structural basis; 2 flavins; domain; nadph; calmodulin; catalysis; reductase; binding (en)
http://linked.open.../riv/klicoveSlovo	structural basis emission spectroscopy reductase flavins redox properties catalysis kinetics molecular modeling photocatalysis domain binding nitric-oxide synthase calmodulin metalloenzymes nadph 2 flavins
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[E7FA14346C34]
http://linked.open...i/riv/nazevZdroje	ChemBioChem
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Nanoparticulate and supramolecular systems for targeted drug delivery
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	10
http://linked.open...iv/tvurceVysledku	Martásek, Pavel Slama-Schwok, A. Beaumont, E. Blanchard-Desce, M. Brochon, JC Deprez, E. Lambry, JC Panda, SP van Faassen, EEH
http://linked.open...ain/vavai/riv/wos	000264168000014
http://linked.open...n/vavai/riv/zamer	Molekulární biologie a patologie buňky za normy a u vybraných klinicky závažných patologických procesů
issn	1439-4227
number of pages	12 (xsd:int)
http://localhost/t...ganizacniJednotka	11110
is http://linked.open...avai/riv/vysledek of	NO Formation by Neuronal NO-Synthase can be Controlled by Ultrafast Electron Injection from a Nanotrigger NO Formation by Neuronal NO-Synthase can be Controlled by Ultrafast Electron Injection from a Nanotrigger NO Formation by Neuronal NO-Synthase can be Controlled by Ultrafast Electron Injection from a Nanotrigger NO Formation by Neuronal NO-Synthase can be Controlled by Ultrafast Electron Injection from a Nanotrigger

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