About: Characterisation of beta amylase alleles in 79 barley varieties with pyrosequencing

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	beta- Amylase is involved in the starch degradation process and therefore influences grain quality. Starch degradation efficiency is dependent on the enzyme thermostability during malting and mashing. Four alleles resulting in different enzyme thermostability are known. These alleles are distinguished by coding single nucleotide polymorphism (cSNP). Pyrosequencing was used for cSNP genotyping of beta--amylase alleles in 79 spring barley varieties by using analyser PSQ MA96 System (Pyrosequencing, Biotage). A new cSNP was revealed by means of Pyrosequencing analysis of sequence flanking cSNP698, thus recognizing a fifth β-amylase allele. Pyrosequencing is a high-throughput, fast, and precise system for barley SNP genotyping. beta- Amylase is involved in the starch degradation process and therefore influences grain quality. Starch degradation efficiency is dependent on the enzyme thermostability during malting and mashing. Four alleles resulting in different enzyme thermostability are known. These alleles are distinguished by coding single nucleotide polymorphism (cSNP). Pyrosequencing was used for cSNP genotyping of beta--amylase alleles in 79 spring barley varieties by using analyser PSQ MA96 System (Pyrosequencing, Biotage). A new cSNP was revealed by means of Pyrosequencing analysis of sequence flanking cSNP698, thus recognizing a fifth β-amylase allele. Pyrosequencing is a high-throughput, fast, and precise system for barley SNP genotyping. (en) beta-amylasa, která je přítomna při rozkladu škrobu má vliv na kvalitu zrna. Účinnost degradace škrobu pak závisí na termostabilitě.Jsou známy 4 alely, které odpovídají různé enzymatické termostabilitě. V tomto příspěvku je popsáno pyrosekvenování pro cSNP alle beta amylasy u 79 odrůd jarního ječmene pomocí analyzátoru PSQ MA96 System (Pyrosequencing, Biotage). Pyrosekvenování v tomto případě přineslo rychlé, přesné a správné výsledky pro vytvoření systému SNP u ječmene. (cs)
Title	Charakterizace beta amylázy v 79 odrůdách ječmene pomocí pyrosequencování (cs) Characterisation of beta amylase alleles in 79 barley varieties with pyrosequencing Characterisation of beta amylase alleles in 79 barley varieties with pyrosequencing (en)
skos:prefLabel	Charakterizace beta amylázy v 79 odrůdách ječmene pomocí pyrosequencování (cs) Characterisation of beta amylase alleles in 79 barley varieties with pyrosequencing Characterisation of beta amylase alleles in 79 barley varieties with pyrosequencing (en)
skos:notation	RIV/00027006:_____/03:2841!RIV08-MZE-00027006
http://linked.open.../vavai/riv/strany	439;447
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(QD1365), Z(MZE-M01-01-02)
http://linked.open...iv/cisloPeriodika	21
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Ovesná, Jaroslava Machová Poláková, Kateřina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	600892
http://linked.open...ai/riv/idVysledku	RIV/00027006:_____/03:2841
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	barley; beta amylase - coding single nucleotide polymorphism - genetic resources - Pyrosequencing (en)
http://linked.open.../riv/klicoveSlovo	barley beta amylase - coding single nucleotide polymorphism - genetic resources - Pyrosequencing
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[33278EA4F6C6]
http://linked.open...i/riv/nazevZdroje	Plant Molecular Biology Reporter
http://linked.open...in/vavai/riv/obor	GE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Selection and Characterisation of Barley Genetic Resources Differing in Kernel End-use Qualities Using Newly Developed DNA Markers
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	2003
http://linked.open...iv/tvurceVysledku	Ovesná, Jaroslava Vaculová, K. Machová-Poláková, Kateřina Laurie, D.
http://linked.open...n/vavai/riv/zamer	Study and Use of Biodiversity, Genetic Mechanisms and New Methods for the Improvement of Biological Potential of Crops and Sustainable Development of Agriculture.
issn	0735-9640
number of pages	9 (xsd:int)

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