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Statements

Subject Item
n2:RIV%2F61988987%3A17110%2F14%3AA1501BT8%21RIV15-MSM-17110___
rdf:type
skos:Concept n17:Vysledek
dcterms:description
Administration of anaesthesia during the daytime is important and may influence specific aspects of in vivo animal experiments. The aim of the present study was to refer to the suitability of the use of ketamine/xylazine anaesthesia in chronobiological studies. Study was performed in ketamine-/xylazine-anaesthetized female Wistar rats ( 100 mg/kg/15 mg/kg, i.m) after adaptation to a light-dark (LD) cycle ( 12 h: 12 h). Heart rate (HR), rectal temperature, ECG parameters, autonomic nervous system activity, acid-base balance and Na+, K+, Ca2+ and Cl(-)plasma concentrations were evaluated in dependence on LD cycle. LD differences were found in the HR, rectal temperature, RR, PQ and QTc intervals, QRS complex, P wave amplitude, in power HF, LF and VLF components, in paO2 and HCO3-, and in Na+, Cl(-)and Ca2+ ions. Ketamine/xylazine anaesthesia may be applicable in chronobiological studies. It does not disturb LD differences, but it can modify them probably without loss of rhythmicity. Administration of anaesthesia during the daytime is important and may influence specific aspects of in vivo animal experiments. The aim of the present study was to refer to the suitability of the use of ketamine/xylazine anaesthesia in chronobiological studies. Study was performed in ketamine-/xylazine-anaesthetized female Wistar rats ( 100 mg/kg/15 mg/kg, i.m) after adaptation to a light-dark (LD) cycle ( 12 h: 12 h). Heart rate (HR), rectal temperature, ECG parameters, autonomic nervous system activity, acid-base balance and Na+, K+, Ca2+ and Cl(-)plasma concentrations were evaluated in dependence on LD cycle. LD differences were found in the HR, rectal temperature, RR, PQ and QTc intervals, QRS complex, P wave amplitude, in power HF, LF and VLF components, in paO2 and HCO3-, and in Na+, Cl(-)and Ca2+ ions. Ketamine/xylazine anaesthesia may be applicable in chronobiological studies. It does not disturb LD differences, but it can modify them probably without loss of rhythmicity.
dcterms:title
Ketamine/xylazine anaesthesia in the chronobiological studies Ketamine/xylazine anaesthesia in the chronobiological studies
skos:prefLabel
Ketamine/xylazine anaesthesia in the chronobiological studies Ketamine/xylazine anaesthesia in the chronobiological studies
skos:notation
RIV/61988987:17110/14:A1501BT8!RIV15-MSM-17110___
n3:aktivita
n13:I
n3:aktivity
I
n3:cisloPeriodika
4
n3:dodaniDat
n5:2015
n3:domaciTvurceVysledku
n8:3419959 n8:1789775
n3:druhVysledku
n4:J
n3:duvernostUdaju
n6:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
24194
n3:idVysledku
RIV/61988987:17110/14:A1501BT8
n3:jazykVysledku
n11:eng
n3:klicovaSlova
ketamine/xylazine; chronobiology; electrophysiology of the heart; acid-base balance; ions; autonomic nervous system
n3:klicoveSlovo
n9:chronobiology n9:ketamine%2Fxylazine n9:electrophysiology%20of%20the%20heart n9:acid-base%20balance n9:ions n9:autonomic%20nervous%20system
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[D1C54A001448]
n3:nazevZdroje
BIOL RHYTHM RES
n3:obor
n14:ED
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
6
n3:rokUplatneniVysledku
n5:2014
n3:svazekPeriodika
45
n3:tvurceVysledku
Švorc, Pavol Jurasova, Z. Bacova, I. Novakova, M. Marossy, A.
n3:wos
000337580100015
s:issn
0929-1016
s:numberOfPages
10
n15:organizacniJednotka
17110