"1"^^ . . . . . . . . "fluorescent protein, linear dichroism, transition dipole moment, polarization microscopy, membrane protein structure, FRET"@en . . "Insights into protein structure from polarization fluorescence microscopy"@en . "fluorescent protein" . "2016-12-31+01:00"^^ . . " polarization microscopy" . . . "V\u00FDzkum struktury protein\u016F prost\u0159ednictv\u00EDm polariza\u010Dn\u00ED fluorescen\u010Dn\u00ED mikroskopie" . . "2014-03-31+02:00"^^ . . . . "http://www.isvav.cz/projectDetail.do?rowId=GA13-10799S"^^ . "GA13-10799S" . "Optick\u00E9 vlastnosti fluorescentn\u00EDch protein\u016F (FP) jsou anisotropick\u00E9, \u010Deho\u017E lze vyu\u017E\u00EDt p\u0159i v\u00FDvoji a pou\u017Eit\u00ED \u0159ady geneticky k\u00F3dovan\u00FDch fluorescentn\u00EDch sond biologick\u00FDch proces\u016F. Anisotropie FP v\u0161ak tak\u00E9 m\u016F\u017Ee (prost\u0159ednictv\u00EDm polariza\u010Dn\u00ED mikroskopie) poskytovat informace o struktu\u0159e protein\u016F, p\u0159\u00EDmo v \u017Eiv\u00FDch bu\u0148k\u00E1ch. Tato mo\u017Enost je v\u0161ak v sou\u010Dasnosti neprozkoum\u00E1na. Navrhujeme proto tuto mezeru zaplnit otestov\u00E1n\u00EDm hypot\u00E9zy, \u017Ee polariza\u010Dn\u00ED mikroskopii lze vyu\u017E\u00EDt k ur\u010Den\u00ED orientace FP v\u016F\u010Di jin\u00FDm \u010D\u00E1stem f\u00FAzn\u00EDho proteinu. Abychom toho dos\u00E1hli, tak 1) ur\u010D\u00EDme orientaci dip\u00F3lov\u00FDch moment\u016F p\u0159echodu (DMP) v\u016F\u010Di bun\u011B\u010Dn\u00E9 membr\u00E1n\u011B u membr\u00E1nov\u00FDch protein\u016F zna\u010Den\u00FDch pomoc\u00ED FP; 2) ur\u010D\u00EDme sm\u011Br DMP v molekul\u00E1ch v\u00FDznamn\u00FDch FP; 3) ur\u010D\u00EDme orientaci FP v konstruktech na b\u00E1zi membr\u00E1nov\u00FDch protein\u016F a porovn\u00E1me ji s dostupn\u00FDmi strukturn\u00EDmi daty. Na\u0161e v\u00FDsledky poskytnou z\u00E1kladn\u00ED informace o fluorescentn\u00EDch proteinech, umo\u017En\u00ED zlep\u0161en\u00ED sou\u010Dasn\u00FDch geneticky k\u00F3dovan\u00FDch fluorescentn\u00EDch sond, a p\u0159isp\u011Bj\u00ED k v\u00FDvoji nov\u00FDch n\u00E1stroj\u016F pro z\u00EDsk\u00E1v\u00E1n\u00ED strukturn\u00EDch informac\u00ED o proteinech." . "2013-02-01+01:00"^^ . . "Optical properties of fluorescent proteins (FPs) are anisotropic. This fact can be used to develop a broad range of genetically encoded probes of biological processes. In principle, FP anisotropy (through polarization microscopy) can also provide information about protein structure, in living cells. This possibility, however, remains unexplored. We want to fill this gap by testing the hypothesis that polarization microscopy can be used to determine the orientation of an FP with respect to other parts of a fusion protein. In order to achieve this goal, we will: 1) determine orientation of transition dipole moments (TDMs) with respect to the cell membrane in FP-labeled membrane proteins; 2) determine TDM direction in molecules of representative FPs; and 3) determine orientation of the FP moiety in several membrane protein constructs and compare the results with available structural data. The results will provide fundamental information about FPs, allow improvements in genetically encoded fluorescent probes, and yield new tools for obtaining protein structure information."@en . " membrane protein structure" . "0"^^ . . . "1"^^ . " transition dipole moment" . . "2015-04-23+02:00"^^ . "0"^^ . "1"^^ . " linear dichroism" .