" evolution" . . " Solanaceae" . "2014-04-23+02:00"^^ . "Strukturn\u00ED a funk\u010Dn\u00ED komponenty rostlinn\u00FDch telomer" . . . . . "Arabidopsis; evolution; chromatin; polyploidy; regulation; Solanaceae; telomere; telomerase"@en . . . "0"^^ . "Structural and functional components of plant telomeres"@en . "2"^^ . "Telomery jsou nukleoproteinov\u00E9 struktury na konc\u00EDch eukaryotick\u00FDch chromosom\u016F, kter\u00E9 jsou udr\u017Eov\u00E1ny aktivitou komplexu telomer\u00E1zy. P\u0159edkl\u00E1dan\u00FD projekt v sob\u011B zahrnuje komplexn\u00ED studie (1) proteinov\u00E9 struktury a interakc\u00ED rostlinn\u00FDch telomer, (2) regulace komplexu telomer\u00E1zy a (3) evoluce struktury genu TERT k\u00F3duj\u00EDc\u00EDho proteinovou podjednotku telomer\u00E1zy. Proteiny \u00FA\u010Dastn\u00EDc\u00ED se tvorby funk\u010Dn\u00EDho komplexu rostlinn\u00E9 telomery budou identifikov\u00E1ny kombinac\u00ED vysokokapacitn\u00EDch proteomick\u00FDch p\u0159\u00EDstup\u016F s vyu\u017Eit\u00EDm modelov\u00FDch rostlin s r\u016Fznou d\u00E9lkou telomer \u2013 Nicotiana tabacum a Arabidopsis thaliana. Kandid\u00E1tn\u00ED proteiny budou n\u00E1sledn\u011B charakterizov\u00E1ny pomoc\u00ED T-DNA inzer\u010Dn\u00EDch lini\u00ED A. thaliana a jejich interakce (DNA-protein, protein-protein) s komponenty telomer budou zkoum\u00E1ny pomoc\u00ED klasick\u00FDch molekul\u00E1rn\u011B-biologick\u00FDch in vitro metod. Studie evoluce struktury telomer\u00E1zov\u00E9ho genu bude roz\u0161\u00ED\u0159ena o polyploidn\u00ED druhy z rodu Nicotiana a \u010Deledi Solanaceae. O\u010Dek\u00E1van\u00E9 v\u00FDstupy projektu p\u0159isp\u011Bj\u00ED k detailn\u00EDmu pochopen\u00ED zp\u016Fsob\u016F a regulace udr\u017Eov\u00E1n\u00ED telomer." . "2"^^ . "1"^^ . . " telomere" . . "2"^^ . "Arabidopsis" . "2017-12-31+01:00"^^ . . . . . . . "http://www.isvav.cz/projectDetail.do?rowId=GA13-06943S"^^ . " regulation" . "2015-04-23+02:00"^^ . . . " polyploidy" . "2013-02-01+01:00"^^ . " chromatin" . "Telomeres are nucleoprotein structures at the ends of eukaryotic chromosomes maintained by telomerase activity. Proposed project involves comprehensive studies of (i) protein structure and interactions of plant telomeres; (ii) telomerase complex regulation; and (iii) evolution of the telomerase protein subunit (TERT) gene structure. Protein components involved in the formation of functional plant telomere complex will be identified by high-throughput proteomic methods using model plants with different telomere length, Nicotiana tabacum and Arabidopsis thaliana. Candidate proteins will be further characterized using A. thaliana T-DNA insertion lines, and their interactions with telomere components (DNA-protein, protein-protein) will be investigated using conventional molecular-biology methods in vitro. Evolution of telomerase gene structure will be extended in polyploid species from the Nicotiana genus and the Solanaceae family. Expected project outcomes will enrich detailed understanding of regulation mechanisms of telomere maintenance."@en . . "GA13-06943S" .