. "0"^^ . . . . . "1"^^ . "2"^^ . " Peptides" . "0"^^ . . "2"^^ . "PBL will be isolated from human blood and peptides will be eluted from B27 molecules of AS patients and healthy control. The peptide profiles will be compared by MALDI-TOF and Edman degradation for pool sequencing. PBL will be isolated from human blood and peptides will be eluted from B27 molecules of AS patients and healthy control.Individual peptides will be sequenced by LC/MS and Q-FTMS. Pools of PBL obtained from several AS diseased and healthy individuals will be tested to examine which individual peptide appears as different,and thus exert a strong representation. Method used for the analysis of individual peptides will be MALDI-TOF. Peptide profiles will be examined from spleen cells of B27 transgenic mice with the ANKENT enthesopathy disease and healthy controls. The overall results will be compared with those obtained from AS patients."@en . "Research report expanding knowledge on pathogenetic process in ankylosing spondylitis."@en . "Autoimmunity; HLA-B27; Peptides; AS"@en . "Autoimmunity" . "2005-08-01+02:00"^^ . "2008-03-05+01:00"^^ . " HLA-B27" . "1A8631" . . . . "http://www.isvav.cz/projectDetail.do?rowId=1A8631"^^ . "Peptidy izolovan\u00E9 z PBL lidsk\u00E9 krve budou eluov\u00E1ny z molekul B27 pacient\u016F s AS, d\u00E1le pacient\u016F s r\u016Fzn\u00FDmi formami B27 asociovan\u00FDch onemocn\u011Bn\u00ED a zdrav\u00FDch kontrol. Peptidov\u00E9 profily budou porovn\u00E1ny metodou MALDI-TOF a Edmanovou degradac\u00ED (pool sequencing). PBL budou izolov\u00E1ny z krve nemocn\u00FDch s AS a zdrav\u00FDch jedinc\u016F. Z\u00EDskan\u00E9 peptidy budou eluov\u00E1ny z molekul B27 v obou vy\u0161et\u0159ovan\u00FDch skupin\u00E1ch. Individu\u00E1ln\u00ED peptidy budou sekvenov\u00E1ny pomoc\u00ED LC/MS a Q-FTMS. Zji\u0161t\u011Bn\u00E9 sekvence peptid\u016F eluovan\u00E9 z kapsy B27 molekuly u nemocn\u00FDch budou srovn\u00E1ny se sekvencemi publikovan\u00FDmi v v B27-peptide REGISTER (Lopez de Castro, Tissue Antigens 2004). Peptidov\u00E9 profily bun\u011Bk ze sleziny B27 transgenn\u00EDch my\u0161\u00ED s ANKENT entesopati\u00ED budou porovn\u00E1ny s kontroln\u00EDmi profily. V\u00FDsledky budou srovn\u00E1ny s \u00FAdaji z\u00EDskan\u00FDmi u pacient\u016F s AS." . . "Sequence of peptides eluted from HLA-B27 molecules in healthy and diseased individuals"@en . . "Sequence peptid\u016F eluovan\u00FDch z HLA-B27 molekul u zdrav\u00FDch a nemocn\u00FDch jedinc\u016F" . . . "2008-12-31+01:00"^^ . . . "2010-04-06+02:00"^^ . . . "V\u011Bdeck\u00FD \u010Dl\u00E1nek p\u0159isp\u00EDvaj\u00EDc\u00ED ke znalostem o mo\u017En\u00FDch patogenetick\u00FDch d\u011Bj\u00EDch ankylozuj\u00EDc\u00ED spondylitidy."@cs . . .