"Development of competitive quantitative real-time PCR method for detection of C.trachomatis and C.pneumoniae DNAs, Generation of external and internal standards by cloning selected DNA fragments into bacterial plasmids Validation of the method (determination of sensitivity and specificity) and evaluation of diagnostic efficiency, based on testing of the defined panels of clinical samples"@en . "5"^^ . . . "2007-08-08+02:00"^^ . "http://www.isvav.cz/projectDetail.do?rowId=1A8259"^^ . . . "0"^^ . . "1A8259" . "V\u00FDvoj metody pr\u016Fkazu DNA C.pneumoniae a C.trachomatis pomoc\u00ED real-time PCR." . "Development of real-time PCR for the detection of DNA Chlamydia trachomatis and Chlamydia pneuminae."@en . "1"^^ . . "Construction and validation of guantitative real-time PCR for simultaneous detection of Chlamydia pneumoniae and C.trachomatis. Construction of recombinat calibr\u00E1tor and internal standard."@en . . . . " Infectious diseases" . "0"^^ . . "V\u00FDvoj metody kvantitativn\u00EDho pr\u016Fkazu DNA Chlamydia pneumoniae a Chlamydia trachomatis metodou kompetitivn\u00ED real-time PCR.1) Navr\u017Een\u00ED primer\u016F a zna\u010Den\u00FDch sond, optimalizace amplifika\u010Dn\u00EDch podm\u00EDnek. 2)P\u0159\u00EDprava vnit\u0159n\u00EDho a vn\u011Bj\u0161\u00EDho standardu pomoc\u00ED klonov\u00E1n\u00ED vybran\u00FDch \u00FAsek\u016F DNA do bakteri\u00E1ln\u00EDch plasmid\u016F.Validace metody (stanoven\u00ED citlivosti, specifity a robustnosti). Ov\u011B\u0159en\u00ED diagnostick\u00E9 \u00FA\u010Dinnosti t\u00E9to metody pomoc\u00ED testov\u00E1n\u00ED definovan\u00FDch soubor\u016F pozitivn\u00EDch a negativn\u00ED vzork\u016F DNA izolovan\u00E9 z r\u016Fzn\u00FDch klinick\u00FDch materi\u00E1l\u016F. Porovn\u00E1n\u00ED z\u00E1chytnosti s jin\u00FDmi dosud pou\u017E\u00EDvan\u00FDmi metodami p\u0159\u00EDm\u00E9ho pr\u016Fkazu chlamydi\u00ED. O\u010Dek\u00E1van\u00FDm p\u0159\u00EDnosem je zp\u0159esn\u011Bn\u00ED a roz\u0161\u00ED\u0159en\u00ED mo\u017Enost\u00ED rychl\u00E9 diagnostiky chlamydiov\u00FDch infekc\u00ED, a t\u00EDm i ke zlep\u0161en\u00ED mo\u017Enost\u00ED jejich \u00FAsp\u011B\u0161n\u00E9 l\u00E9\u010Dby a epidemiologick\u00E9 prevence." . " Chlamydia pneumoniae" . . . "Konstrukce a validace real-time PCR pro kvantitativn\u00ED pr\u016Fkaz Chlamydia pneumoniae a C. trachomatis. Konstrukce kalibr\u00E1toru a vnit\u0159\u00EDn\u00EDho standardu."@cs . . " Diagnosis" . . . . "Chlamydia trachomatis" . "Chlamydia trachomatis; Chlamydia pneumoniae; Diagnosis; Infectious diseases; Real-time PCR"@en . . . . "5"^^ .