" oncogenes" . "melanoma" . . "2007-08-08+02:00"^^ . . . "Alteration of gene expretion and signal transduction in malignant melanoma cells. Relationship to changes of stromal micro-enviroment."@en . "1A8245" . . . . . . . " angiogenesis" . . . . . "http://www.isvav.cz/projectDetail.do?rowId=1A8245"^^ . " transformation" . "0"^^ . . "0"^^ . "Alterace genov\u00E9 exprese a sign\u00E1ln\u00ED transdukce v bu\u0148k\u00E1ch malign\u00EDho melanomu. Vztah ke zm\u011Bn\u00E1m strom\u00E1ln\u00EDho mikroprost\u0159ed\u00ED." . "We proved significant increase in nestin expression in melanoma groups, especialla in nodular melanomas, where nestin was localized mainly in the peripheral, ivasive areas of the tumor mass."@en . "1"^^ . . "The aim of the study is to analyse VEGF A,B,C and VEGFR 1,2, 3 expression, expression of factors modulating stromal environmental fenotype-PDGF, EGF, c-Myc and expression of STAT1, STAT3 and nestin in MM and stromal cells. To evaluate prognostic importance of factors mentioned above with clinical state, sentinel lymph node involvement, eventually with presence of distant metastases and with patients' survival. To correlate the results with serological prognostic marker - MIA (melanocytes inhibiting antigen). We plan to examine about 250 MM cases, 50 dysplastic pigment nevi and 100 benign nevi as a control group. Immunocytochemistry and immunohistochemistry and Western blot analysis will be used for detection."@en . "Prok\u00E1z\u00E1no v\u00FDznamn\u00E9 zv\u00FD\u0161en\u00ED exprese nestinu u jednotliv\u00FDch typ\u016F melanom\u016F, zejm\u00E9na u melanom\u016F nodul\u00E1rn\u00EDch. Protein byl lokalizov\u00E1n zvl\u00E1\u0161t\u011B v perifern\u00EDch, invazivn\u00EDch \u010D\u00E1stech tumoru."@cs . " microenviroment" . . . "C\u00EDlem projektu je v bu\u0148k\u00E1ch MM a strom\u00E1ln\u00EDch elementech analyzovat expresi VEGF A,B,C a VEGFR 1,2,3, expresi faktor\u016F moduluj\u00EDc\u00EDch fenotyp strom\u00E1ln\u00EDho prost\u0159ed\u00ED- PDGF, EGF, bFGF, c-Myc, sledovat expresi STAT1, STAT3, FAS-L a nestinu. Posoudit prognostick\u00FD v\u00FDznam exprese v\u00FD\u0161e uveden\u00FDch znak\u016F porovn\u00E1n\u00EDm s klinick\u00FDm obrazem, pozitivitou sentinelov\u00FDch uzlin, p\u0159\u00EDpadn\u011B p\u0159\u00EDtomnost\u00ED metast\u00E1z v jin\u00FDch org\u00E1nech a s p\u0159e\u017Eit\u00EDm pacient\u016F. Korelovat v\u00FD\u0161e uveden\u00E9 v\u00FDsledky se serologick\u00FDm ukazatelem progrese MM-MIA (melanocyty inhibuj\u00EDc\u00ED antigen). Pl\u00E1nujeme vy\u0161et\u0159it soubor asi 250 MM, 50 dysplastick\u00FDch n\u00E9v\u016F a 100 benign\u00EDch pigmentov\u00FDch n\u00E9v\u016F jako kontroln\u00ED skupiny. Pro experimenty pou\u017Eijeme imunocytochemickou a imunohistochemickou detekci a Western blot anal\u00FDzu." . . . "6"^^ . " fibroblasts" . . "6"^^ . . "melanoma; angiogenesis; microenviroment; oncogenes; transformation; fibroblasts; immunohistochemistry"@en .