"To characterize the human cytochrome P450 forms involved in dimemorfan oxidation (DFO), human liver microsomes, and recombinant P450s were investigated. Liquid chromatography-mass spectral analysis suggested that metabolite (M)1 ([M+H](+) m/z at 272.200) and M2 ([M+H](+) m/z at 242.190) were d-3-hydroxymethyl-N-methylmorphinan and d-3-methylmorphinan, respectively. Kinetic analyses of microsomal DFO showed that the substrate concentration showing a half-maximal velocity (S(50)) of M1 formation was less than that of M2. Microsomal M1 and M2 formation activities correlated significantly with the CYP2D6 marker, dextromethorphan O-demethylation activity. The results indicated the participation of multiple P450 forms in DFO."@en . "metabolism; dimemorfan; cytochrome P450"@en . . . "Chi, C. W." . "0022-3549" . "[3AA1C0457006]" . "RIV/75010330:_____/10:00008753" . . . "The oxidative metabolism of dimemorfan by human cytochrome P450 enzymes" . "000274114100043" . "Chou, Y.C." . "The oxidative metabolism of dimemorfan by human cytochrome P450 enzymes" . "V" . "Krausz, K. W." . "RIV/75010330:_____/10:00008753!RIV11-MZ0-75010330" . . "Chung, Y. T." . "1"^^ . "Lee, C. H." . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Journal of pharmaceutical sciences" . "The oxidative metabolism of dimemorfan by human cytochrome P450 enzymes"@en . . "99" . . . "Liu, T.Y." . "11"^^ . . . "Gelboin, H. V." . "Chau, G.Y." . "15"^^ . . . . . "Ueng, Y.F." . "277875" . "To characterize the human cytochrome P450 forms involved in dimemorfan oxidation (DFO), human liver microsomes, and recombinant P450s were investigated. Liquid chromatography-mass spectral analysis suggested that metabolite (M)1 ([M+H](+) m/z at 272.200) and M2 ([M+H](+) m/z at 242.190) were d-3-hydroxymethyl-N-methylmorphinan and d-3-methylmorphinan, respectively. Kinetic analyses of microsomal DFO showed that the substrate concentration showing a half-maximal velocity (S(50)) of M1 formation was less than that of M2. Microsomal M1 and M2 formation activities correlated significantly with the CYP2D6 marker, dextromethorphan O-demethylation activity. The results indicated the participation of multiple P450 forms in DFO." . "Sou\u010Dek, Pavel" . "The oxidative metabolism of dimemorfan by human cytochrome P450 enzymes"@en . "Wang, S. Y." . "2" .