. "Prague" . "Kladno" . . . "3"^^ . "Mr\u00E1zek, J." . "176503" . "Using genetic engineering methods for detection lower fungi in digestive tract of primates" . . . . . "Instuments and Methods for Biology and Medicine 2012" . . "21460" . . "978-80-01-05119-1" . "The aim of this work is detection of eukaryotic organisms-fungi-in digestive tract of primates. The presence of fungi in digestive tract of primates has not been studied yet. The first part of project is to establish appropriate methods of genetic engineering and optimize these methods. Control samples of mouflon faeces are used to establish methodology, because mouflon digestive tract contains fungi, so results of our testing experiments for fungal detection should come outpositive. DNA is isolated from control samples of mouflon faeces, products of DNA amplification by PCR are analyzed by agarose gel electrophoresis.The main goal is setting PCR condition to increase sensitivity of detection of fungal ITS regions. The next step is sequencing and comparison of sequences in the nucleotide database to find typesof fungi. Final testing and experiments at gorilla faeces will take place in future."@en . "The aim of this work is detection of eukaryotic organisms-fungi-in digestive tract of primates. The presence of fungi in digestive tract of primates has not been studied yet. The first part of project is to establish appropriate methods of genetic engineering and optimize these methods. Control samples of mouflon faeces are used to establish methodology, because mouflon digestive tract contains fungi, so results of our testing experiments for fungal detection should come outpositive. DNA is isolated from control samples of mouflon faeces, products of DNA amplification by PCR are analyzed by agarose gel electrophoresis.The main goal is setting PCR condition to increase sensitivity of detection of fungal ITS regions. The next step is sequencing and comparison of sequences in the nucleotide database to find typesof fungi. Final testing and experiments at gorilla faeces will take place in future." . "Jaro\u0161\u00EDkov\u00E1, Ta\u0165\u00E1na" . . "2"^^ . . . "4"^^ . "Using genetic engineering methods for detection lower fungi in digestive tract of primates"@en . . . "RIV/68407700:21460/12:00202752!RIV13-MSM-21460___" . "Using genetic engineering methods for detection lower fungi in digestive tract of primates" . "eukaryotic organisms-fungi-in; digestive tract; primates; genetic engineering; , DNA; , PCR condition"@en . . "[B63A5D383CB4]" . "RIV/68407700:21460/12:00202752" . . "Using genetic engineering methods for detection lower fungi in digestive tract of primates"@en . "Fliegerov\u00E1, K." . . . "P(EE.2.3.20.0092)" . . . . "Hamrov\u00E1, Jitka" . "\u010Cesk\u00E9 vysok\u00E9 u\u010Den\u00ED technick\u00E9 v Praze" . . "2012-05-29+02:00"^^ .