"21460" . "Kompenzace a ohodnocen\u00ED chyb 3D rekonstrukce z konfok\u00E1ln\u00EDch mikroskopick\u00FDch obraz\u016F"@cs . . . . "Br\u016F\u017Ea, P." . . "2"^^ . "Kompenzace a ohodnocen\u00ED chyb 3D rekonstrukce z konfok\u00E1ln\u00EDch mikroskopick\u00FDch obraz\u016F"@cs . "Heidelberg" . "Jan\u00E1\u010Dek, J." . "Compensation and Evaluation of Errors of 3D Reconstructions from Confocal Microscopic Images"@en . . . "EMC 2008 - 14th European Microscopy Congress" . . . . "360726" . . "Kocandov\u00E1, L." . . "Pro dosa\u017Een\u00ED objemov\u00E9 rekonstrukce elasticky registrujeme konfok\u00E1ln\u00ED obrazy z n\u00E1sledn\u00FDch fyzick\u00FDch \u0159ez\u016F. Vyu\u017Eit\u00EDm obraz\u016F z USB sv\u011Bteln\u00E9ho mikroskopu se p\u0159esnost registrace zv\u00FD\u0161\u00ED. Postup je n\u00E1sleduj\u00EDc\u00ED: Nejd\u0159\u00EDve se registruj\u00ED konfok\u00E1ln\u00ED data v\u016F\u010Di obraz\u016Fm z USB mikroskopu a n\u00E1sledn\u011B se registruj\u00ED ji\u017E p\u0159izp\u016Fsoben\u00E1 konfok\u00E1ln\u00ED data v\u016F\u010Di sob\u011B. V\u00FD\u0161e popsan\u00FDm zp\u016Fsobem dosahujeme dvoj\u00EDho c\u00EDle. Jednak kvantifikujeme m\u00EDru deformac\u00ED a t\u00EDm chybu rekonstrukce a jednak na z\u00E1klad\u011B znalosti t\u00E9to chyby docilujeme p\u0159esn\u011Bj\u0161\u00ED objemov\u00E9 rekonstrukce."@cs . "\u010Capek, Martin" . . "6"^^ . "Compensation and Evaluation of Errors of 3D Reconstructions from Confocal Microscopic Images"@en . . "[08D5727C767B]" . . "P(GA102/08/0691), P(IAA100110502), P(IAA500200510), P(LC06063), Z(AV0Z50110509), Z(MSM6840770012)" . . "We reconstruction results using a 17-day-old embryo of a laboratory Norway rat here. Ten confocal images of physical slices of the embryo were aligned with corresponding images of these slices captured by the USB light microscope by elastic registration. We measured areas of embryo slices from confocal data both before and after alignment and also from USB light microscope data. The measurements revealed partial deformations of cut physical slices (decreasing / increasing of areas measured from original confocal data with respect to USB light microscope data up to 92.7% / 105.3%), but an extensive one as well (decreasing the area of the embryo slice in confocal data to 55% only). However, even this dramatic deformation was successfully compensated by elastic alignment. Thus, our method for compensation of deformations using accessorial data from the USB light microscope turns up to be efficient and providing us with realistic visualization of biological structures under study."@en . . . . "RIV/68407700:21460/08:12145530!RIV09-MSM-21460___" . "978-3-540-85154-7" . "Compensation and Evaluation of Errors of 3D Reconstructions from Confocal Microscopic Images" . "Aachen" . . "Kub\u00EDnov\u00E1, L." . "1"^^ . "Compensation and Evaluation of Errors of 3D Reconstructions from Confocal Microscopic Images" . "2008-09-01+02:00"^^ . . . "Vagnerov\u00E1, R." . "RIV/68407700:21460/08:12145530" . "3D reconstruction; confocal microscopy; error"@en . "We reconstruction results using a 17-day-old embryo of a laboratory Norway rat here. Ten confocal images of physical slices of the embryo were aligned with corresponding images of these slices captured by the USB light microscope by elastic registration. We measured areas of embryo slices from confocal data both before and after alignment and also from USB light microscope data. The measurements revealed partial deformations of cut physical slices (decreasing / increasing of areas measured from original confocal data with respect to USB light microscope data up to 92.7% / 105.3%), but an extensive one as well (decreasing the area of the embryo slice in confocal data to 55% only). However, even this dramatic deformation was successfully compensated by elastic alignment. Thus, our method for compensation of deformations using accessorial data from the USB light microscope turns up to be efficient and providing us with realistic visualization of biological structures under study." . "Springer-Verlag" . .