"A confocal laser scanning microscope (CLSM) captures images from a biological specimen in different depths and provides one with a stack of precisely registered fluorescent images. However, image intensities suffer from light loss distortions showing contrast and brightness degradation of images with depth. This effect causes problems in subsequent analysis and segmentation of biological objects. Therefore, we developed a post-processing method to correct this problem. It is a two step algorithm. In the first step the algorithm determines a standard histogram of the stack of images and in the second step histograms of individual images are warped according to this standard histogram. The algorithm results in more stable brightness and contrast of images through the stack." . "Kompenzace \u00FAtlumu jasu a kontrastu obraz\u016F s hloubkou zaost\u0159en\u00ED v konfok\u00E1ln\u00ED mikroskopii"@cs . . . "21460" . . . "\u010Capek, Martin" . "Compensation of the Contrast and Brightness Attenuation with Depth in Confocal Microscopy"@en . . "[7B3E6C33F4D8]" . "Dvoustup\u0148ov\u00FD algoritmus pro kompenzaci jasu a kontrastu s hloubkou zaost\u0159en\u00ED v konfok\u00E1ln\u00ED mikroskopii."@cs . "brightness correction; contrast correction; histogram warping"@en . . "H\u00E1na, Karel" . . "Proceedings Spring Conference on Computer Graphics" . . "4"^^ . . "Compensation of the Contrast and Brightness Attenuation with Depth in Confocal Microscopy" . . "Compensation of the Contrast and Brightness Attenuation with Depth in Confocal Microscopy"@en . . "Smr\u010Dka, Pavel" . . "Kub\u00EDnov\u00E1, L." . "515969" . "Bratislava" . "Compensation of the Contrast and Brightness Attenuation with Depth in Confocal Microscopy" . . . "2005-05-12+02:00"^^ . "RIV/68407700:21460/05:12108532" . . . "80-223-2057-9" . "3"^^ . . . "4"^^ . . "Univerzita Komensk\u00E9ho v Bratislave" . "Kompenzace \u00FAtlumu jasu a kontrastu obraz\u016F s hloubkou zaost\u0159en\u00ED v konfok\u00E1ln\u00ED mikroskopii"@cs . . . . . "P(IAA100110502), P(IAA600110507), P(KJB6011309), Z(AV0Z50110509), Z(MSM6840770012)" . "A confocal laser scanning microscope (CLSM) captures images from a biological specimen in different depths and provides one with a stack of precisely registered fluorescent images. However, image intensities suffer from light loss distortions showing contrast and brightness degradation of images with depth. This effect causes problems in subsequent analysis and segmentation of biological objects. Therefore, we developed a post-processing method to correct this problem. It is a two step algorithm. In the first step the algorithm determines a standard histogram of the stack of images and in the second step histograms of individual images are warped according to this standard histogram. The algorithm results in more stable brightness and contrast of images through the stack."@en . "RIV/68407700:21460/05:12108532!RIV09-MSM-21460___" . "Budmerice" .