"Nov\u00E1k, Luk\u00E1\u0161" . . "21230" . . "1"^^ . "S" . . "RIV/68407700:21230/07:03132940!RIV09-MSM-21230___" . "Catching Bird Flu in a Droplet" . "[99BC7DB1683B]" . . "Detekce pta\u010D\u00ED ch\u0159ipky"@cs . "5"^^ . "Catching Bird Flu in a Droplet" . "10" . "bird flu detection"@en . . "1078-8956" . "13" . . "Detekce pta\u010D\u00ED ch\u0159ipky"@cs . . . "It is assumed that a timely mass administration of antiviral drugs, backed by quarantines and social distancing, could contain a nascent influenza epidemic at its source, provided that the first clusters of cases were localized within a short time. However, effective routine surveillance may be impossible in countries lacking basic public health resources. For a global containment strategy to be successful, low-cost, easy-to-use handheld units that permit decentralized testing would be vital. Here we present a microfluidic platform that can detect the highly pathogenic avian influenza virus H5N1 in a throat swab sample by using magnetic forces to manipulate a free droplet containing superparamagnetic particles. In a sequential process, the viral RNA is isolated, purified, preconcentrated by 50,000% and subjected to ultrafast real-time RT-PCR. Compared to commercially available tests, the bioassay is equally sensitive and is 440% faster and 2,000-5,000% cheaper." . "Catching Bird Flu in a Droplet"@en . "Neu\u017Eil, P." . "000249980200044" . "Pipper, J." . "Detekce pta\u010D\u00ED ch\u0159ipky (virus H5N1)."@cs . "RIV/68407700:21230/07:03132940" . . "Nature Medicine" . . "Ng, L.F.-P." . "It is assumed that a timely mass administration of antiviral drugs, backed by quarantines and social distancing, could contain a nascent influenza epidemic at its source, provided that the first clusters of cases were localized within a short time. However, effective routine surveillance may be impossible in countries lacking basic public health resources. For a global containment strategy to be successful, low-cost, easy-to-use handheld units that permit decentralized testing would be vital. Here we present a microfluidic platform that can detect the highly pathogenic avian influenza virus H5N1 in a throat swab sample by using magnetic forces to manipulate a free droplet containing superparamagnetic particles. In a sequential process, the viral RNA is isolated, purified, preconcentrated by 50,000% and subjected to ultrafast real-time RT-PCR. Compared to commercially available tests, the bioassay is equally sensitive and is 440% faster and 2,000-5,000% cheaper."@en . "6"^^ . "Inoue, M." . "Catching Bird Flu in a Droplet"@en . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Zhang, Y." . . "412747" .