. "3"^^ . . . . "Pitfalls in Assessing Specific and Affinity of Non-Transferrin-Bound from Uptake." . . "Analytical Biochemistry" . "[E57AB0549305]" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Pitfalls in Assessing Specific and Affinity of Non-Transferrin-Bound from Uptake."@en . . "N/A"@en . "778497" . "Pitfalls in Assessing Specific and Affinity of Non-Transferrin-Bound from Uptake." . . "Z(AV0Z5052915)" . "Kriegerbeckov\u00E1, Karin" . "RIV/68378050:_____/98:23000086!RIV/2003/AV0/A23003/N" . "Brunner-Dopper, L." . "261" . . "1" . . . . . "0003-2697" . "When studying non-transferrin-bound iron (NTBI) uptake by mammalian cells in the high-affinity range (micromolar and below), employment of strong chelators such as NTA can lead to kinetic artifacts due to inhibition by excess chelator. This can be avoided by using citrate at an excess concentration above as a little as1,5-fold over iron without danger of precipitation if balanced culture media are used as incubation mediumfor NTBI uptake."@en . "Pitfalls in Assessing Specific and Affinity of Non-Transferrin-Bound from Uptake."@en . "RIV/68378050:_____/98:23000086" . . "When studying non-transferrin-bound iron (NTBI) uptake by mammalian cells in the high-affinity range (micromolar and below), employment of strong chelators such as NTA can lead to kinetic artifacts due to inhibition by excess chelator. This can be avoided by using citrate at an excess concentration above as a little as1,5-fold over iron without danger of precipitation if balanced culture media are used as incubation mediumfor NTBI uptake." . "4"^^ . "0"^^ . "0"^^ . "Goldenberg, H." . "Kov\u00E1\u0159, Jan" . "128;130" . . "2"^^ .