. . . "Histochemistry and Cell Biology" . "nuclear actin; transcription; mitosis; actin-related protein 3; cofilin"@en . . . "[6FBEC0DA4EB6]" . "6"^^ . "000339963300003" . "RIV/68378050:_____/14:00434269" . . "Nuclear actin filaments recruit cofilin and actin-related protein 3, and their formation is connected with a mitotic block"@en . "RIV/68378050:_____/14:00434269!RIV15-GA0-68378050" . "DE - Spolkov\u00E1 republika N\u011Bmecko" . "14"^^ . . . . . . "Uli\u010Dn\u00E1, L\u00EDvia" . . "33221" . . "0948-6143" . "I, P(ED1.1.00/02.0109), P(GAP305/11/2232), P(LD12063)" . . . . "Nuclear actin filaments recruit cofilin and actin-related protein 3, and their formation is connected with a mitotic block" . . "10.1007/s00418-014-1243-9" . "2" . "Hoz\u00E1k, Pavel" . . "142" . . "Kalasov\u00E1, Ilona" . "Nuclear actin filaments recruit cofilin and actin-related protein 3, and their formation is connected with a mitotic block"@en . "Although actin monomers polymerize into filaments in the cytoplasm, the form of actin in the nucleus remains elusive. We searched for the form and function of \u03B2-actin fused to nuclear localization signal and to enhanced yellow fluorescent protein (EN-actin). Our results reveal that EN-actin is either dispersed in the nucleoplasm (homogenous EN-actin) or forms bundled filaments in the nucleus (EN-actin filaments). Formation of such filaments was not connected with increased EN-actin levels. Among numerous actin-binding proteins tested, only cofilin is recruited to the EN-actin filaments. Overexpression of EN-actin causes increase in the nuclear levels of actin-related protein 3 (Arp3). Although Arp3, a member of actin nucleation complex Arp2/3, is responsible for EN-actin filament nucleation and bundling, the way cofilin affects nuclear EN-actin filaments dynamics is not clear. While cells with homogenous EN-actin maintained unaffected mitosis during which EN-actin re-localizes to the plasma membrane, generation of nuclear EN-actin filaments severely decreases cell proliferation and interferes with mitotic progress. The introduction of EN-actin manifests in two mitotic-inborn defects\u2014formation of binucleic cells and generation of micronuclei\u2014suggesting that cells suffer aberrant cytokinesis and/or impaired chromosomal segregation. In interphase, nuclear EN-actin filaments passed through chromatin region, but do not co-localize with either chromatin remodeling complexes or RNA polymerases I and II. Surprisingly presence of EN-actin filaments was connected with increase in the overall transcription levels in the S-phase by yet unknown mechanism. Taken together, EN-actin can form filaments in the nucleus which affect important cellular processes such as transcription and mitosis." . "Nuclear actin filaments recruit cofilin and actin-related protein 3, and their formation is connected with a mitotic block" . "Yamazaki, S." . . . . "4"^^ . "Harata, M." . "Kalendov\u00E1, Al\u017Eb\u011Bta" . "Although actin monomers polymerize into filaments in the cytoplasm, the form of actin in the nucleus remains elusive. We searched for the form and function of \u03B2-actin fused to nuclear localization signal and to enhanced yellow fluorescent protein (EN-actin). Our results reveal that EN-actin is either dispersed in the nucleoplasm (homogenous EN-actin) or forms bundled filaments in the nucleus (EN-actin filaments). Formation of such filaments was not connected with increased EN-actin levels. Among numerous actin-binding proteins tested, only cofilin is recruited to the EN-actin filaments. Overexpression of EN-actin causes increase in the nuclear levels of actin-related protein 3 (Arp3). Although Arp3, a member of actin nucleation complex Arp2/3, is responsible for EN-actin filament nucleation and bundling, the way cofilin affects nuclear EN-actin filaments dynamics is not clear. While cells with homogenous EN-actin maintained unaffected mitosis during which EN-actin re-localizes to the plasma membrane, generation of nuclear EN-actin filaments severely decreases cell proliferation and interferes with mitotic progress. The introduction of EN-actin manifests in two mitotic-inborn defects\u2014formation of binucleic cells and generation of micronuclei\u2014suggesting that cells suffer aberrant cytokinesis and/or impaired chromosomal segregation. In interphase, nuclear EN-actin filaments passed through chromatin region, but do not co-localize with either chromatin remodeling complexes or RNA polymerases I and II. Surprisingly presence of EN-actin filaments was connected with increase in the overall transcription levels in the S-phase by yet unknown mechanism. Taken together, EN-actin can form filaments in the nucleus which affect important cellular processes such as transcription and mitosis."@en . .