"RIV/68378050:_____/14:00431748!RIV15-GA0-68378050" . "Sulimenko, Vadym" . . "Sulimenko, Tetyana" . "Freeze-drying; Microtubules; Stability; Trehalose; Tubulin"@en . "Stabilization of Protein by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin"@en . "The chapter 32 covers an elegant method for lyphilization of tubulin. Authors describe step by step the protocol for preparation of freeze-drying (lyophilizated) sample of tubulin. Tubulin is heterodimeric protein that composes microtubules as part of cytoskeleton of the cell. However, tubulin is a thermolabile protein that rapidly converts into non-polymerazing state. For this reason it is usually stored at -80\u00B0C to preserve its conformation and polymerization properties. In this chapter authors describe a method for freeze-drying of assembly-competent tubulin in the presence of nonreducing sugar trehalose and methods enabling the evaluation of tubulin functions in rehydrated samples. The chapter also includes images documenting the effect of trehalose on the functionality of the rehydrated tubulin suitable for in vitro studies."@en . "978-1-62703-976-5" . . "555"^^ . "[699FB0FAF0FB]" . "Stabilization of Protein by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin"@en . . . . . . "4"^^ . . "4"^^ . "Protein Downstream Processing" . . . . "Dr\u00E1ber, Pavel" . . "Humana Press INC" . . "New York" . . . . "46989" . "Stabilization of Protein by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin" . "RIV/68378050:_____/14:00431748" . . . "Dr\u00E1berov\u00E1, Eduarda" . . "I, P(GAP302/10/1701), P(GAP302/12/1673), P(GPP302/11/P709), P(LH12050)" . "16"^^ . . . . . "10.1007/978-1-62703-977-2_32" . "Stabilization of Protein by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin" . "The chapter 32 covers an elegant method for lyphilization of tubulin. Authors describe step by step the protocol for preparation of freeze-drying (lyophilizated) sample of tubulin. Tubulin is heterodimeric protein that composes microtubules as part of cytoskeleton of the cell. However, tubulin is a thermolabile protein that rapidly converts into non-polymerazing state. For this reason it is usually stored at -80\u00B0C to preserve its conformation and polymerization properties. In this chapter authors describe a method for freeze-drying of assembly-competent tubulin in the presence of nonreducing sugar trehalose and methods enabling the evaluation of tubulin functions in rehydrated samples. The chapter also includes images documenting the effect of trehalose on the functionality of the rehydrated tubulin suitable for in vitro studies." . .