"[CED3A720B77E]" . "3"^^ . "RNA interference (RNAi) is an evolutionary conserved gene-silencing pathway that can be efficiently utilized as a tool to study gene function. RNAi is initiated by long double-stranded RNAs (dsRNAs), which are processed into small duplexes called small-interfering RNAs (siRNAs). In turn, these duplexes target mRNAs for degradation in a sequence-specific manner. Mouse oocytes, unlike most mammalian cell types, lack an interferon response to long dsRNA. Moreover, they are a rare example of a mammalian cell type with a robust endogenous RNAi pathway. For these reasons microinjection of either long dsRNAs or siRNAs results in efficient, sequence-specific gene silencing. Here, we describe a protocol for preparation and microinjection of long dsRNA into mouse oocytes."@en . "311"^^ . "RNAi-based methods for gene silencing in mouse oocytes"@en . "I" . . . . "RNA interference (RNAi) is an evolutionary conserved gene-silencing pathway that can be efficiently utilized as a tool to study gene function. RNAi is initiated by long double-stranded RNAs (dsRNAs), which are processed into small duplexes called small-interfering RNAs (siRNAs). In turn, these duplexes target mRNAs for degradation in a sequence-specific manner. Mouse oocytes, unlike most mammalian cell types, lack an interferon response to long dsRNA. Moreover, they are a rare example of a mammalian cell type with a robust endogenous RNAi pathway. For these reasons microinjection of either long dsRNAs or siRNAs results in efficient, sequence-specific gene silencing. Here, we describe a protocol for preparation and microinjection of long dsRNA into mouse oocytes." . "978-1-62703-190-5" . "RNAi-based methods for gene silencing in mouse oocytes" . "New York" . "RNAi-based methods for gene silencing in mouse oocytes" . "RIV/68378050:_____/13:00423294" . . "RNAi; dsRNA; mouse oocyte; microinjection; knockdown"@en . "Stein, P." . "Methods in Molecular Biology, 957" . . . . "Schultz, R. M." . "10.1007/978-1-62703-191-2" . . "RNAi-based methods for gene silencing in mouse oocytes"@en . . . . "Svoboda, Petr" . "Mammalian Oocyte Regulation" . . . "Humana Press" . "17"^^ . "RIV/68378050:_____/13:00423294!RIV14-AV0-68378050" . "103126" . . . . . "1"^^ .