"accelerated rejection; corneal transplantation; dendritic cells; regraft; T-cell memory; transgenic mouse"@en . "4" . "9"^^ . . . . . "The high-risk corneal regraft model: a justification for tissue matching in humans"@en . "1"^^ . "Kuffova, L." . . "RIV/68378050:_____/13:00392147!RIV14-AV0-68378050" . "0934-0874" . "26" . . . "The high-risk corneal regraft model: a justification for tissue matching in humans" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . . "Transplant International" . "Klaska, I." . . "The high-risk corneal regraft model: a justification for tissue matching in humans"@en . "Models of high-risk corneal graft rejection involve neovascularization induced via innate immune responses, e.g., suture-mediated trauma. We describe a model of high-risk corneal graft rejection using corneal graft donor-recipient pairing based on a single-antigen disparity. Donor corneas from transgenic mice on B10.BR (H-2k) background, in which hen-egg lysozyme (HEL) as a membrane-bound antigen (mHEL) was expressed under the major histocompatibility complex (MHC) Class I promoter (KLK-mHEL, H-2k), were transplanted into wild type B10.BR recipient mice. Unmanipulated wild type recipient mice rejected KLK-mHEL grafts (39%) slowly over 5060days. Graft rejection incidence was maximized (100%) and tempo accelerated (27days) by priming with HEL-pulsed syngeneic dendritic cells and less so by increasing T-cell precursor frequency. Rejection also reached maximum levels (100%) and tempo (38days) when mice which had rejected a first graft (rejectors') were regrafted, and was associated with induction of HEL-specific memory T cells. In contrast, acceptors' rejected a second graft at rates and tempo similar to naive mice. These data reveal the importance of (i) donor MHC antigens as alloantigens for indirect recognition, (ii) alloantigen-specific memory in high-risk graft rejection involving regrafts, and (iii) suggest a role for tissue matching in human corneal graft to avoid sensitization to donor MHC antigens."@en . "77435" . . . "Cornall, R. J." . "Vitova, A." . "000316633100018" . . . "Forrester, J. V." . "[B42250DC52D4]" . . . "I" . "Models of high-risk corneal graft rejection involve neovascularization induced via innate immune responses, e.g., suture-mediated trauma. We describe a model of high-risk corneal graft rejection using corneal graft donor-recipient pairing based on a single-antigen disparity. Donor corneas from transgenic mice on B10.BR (H-2k) background, in which hen-egg lysozyme (HEL) as a membrane-bound antigen (mHEL) was expressed under the major histocompatibility complex (MHC) Class I promoter (KLK-mHEL, H-2k), were transplanted into wild type B10.BR recipient mice. Unmanipulated wild type recipient mice rejected KLK-mHEL grafts (39%) slowly over 5060days. Graft rejection incidence was maximized (100%) and tempo accelerated (27days) by priming with HEL-pulsed syngeneic dendritic cells and less so by increasing T-cell precursor frequency. Rejection also reached maximum levels (100%) and tempo (38days) when mice which had rejected a first graft (rejectors') were regrafted, and was associated with induction of HEL-specific memory T cells. In contrast, acceptors' rejected a second graft at rates and tempo similar to naive mice. These data reveal the importance of (i) donor MHC antigens as alloantigens for indirect recognition, (ii) alloantigen-specific memory in high-risk graft rejection involving regrafts, and (iii) suggest a role for tissue matching in human corneal graft to avoid sensitization to donor MHC antigens." . "Hol\u00E1\u0148, Vladim\u00EDr" . . "6"^^ . "RIV/68378050:_____/13:00392147" . "The high-risk corneal regraft model: a justification for tissue matching in humans" . "10.1111/tri.12055" . .