. "gamma-tubulin; microtubules; NSCLC"@en . "12"^^ . "[83CE3FFAE017]" . "Giordano, A." . . "WWe and others have previously shown that increased expression and altered compartmentalization of \u03B3-tubulin may contribute to tumorigenesis and tumor progression (J. Cell Physiol. 2009;223:519-529; Cancer Biol. Ther. 2010;9:66-76). Here we have determined by immunohistochemistry the localization and cellular distribution of \u03B3-tubulin in clinical tissue samples from 109 non-small cell lung cancer (NSCLC) cases. The expression and distribution of \u03B3-tubulin protein and transcripts was also determined in the NSCLC tumor cell lines NCI-H460 (HTB-177) and NCI-H69 (HTB-119) by immunocytochemistry, quantitative immunoblotting and reverse transcription quantitative real-time PCR (RT-qPCR). Polyclonal and monoclonal anti-peptide antibodies recognizing epitopes in the C- or N-terminal domains of \u03B3-tubulins and human gene-specific primers for \u03B3-tubulins 1 (TUBG1) and 2 (TUBG2) were used. In non-neoplastic cells of the airway epithelium in situ, \u03B3-tubulin exhibited predominantly apical surface and pericentriolar localizations. In contrast, markedly increased, albeit heterogeneous and variously prominent \u03B3-tubulin immunoreactivity was detected in clinical tumor specimens and in the NCI-H460 and NCI-H69 cell lines, where tumor cells exhibited overlapping multi-punctate and diffuse patterns of localization. Co-expression of \u03B3-tubulin and Ki-67 (MIB-1) was detected in a population of proliferating tumor cells. A statistically significant increase of \u03B3-tubulin expression was found in Stage III compared to lesser stage tumors (p<0.001 v. Stages I/II) regardless of histological subtype or grade. By quantitative immunoblotting NCI-H460 and NCI-H69 cells expressed higher levels of \u03B3-tubulin protein compared to small airway epithelial cells (SAEC). In both tumor cell lines increase in TUBG1 and TUBG2 transcripts was detected by RT-qPCR. Our results reveal for the first time an increased expression of \u03B3-tubulin in lung cancer." . . . . . "Riga, D." . . "WWe and others have previously shown that increased expression and altered compartmentalization of \u03B3-tubulin may contribute to tumorigenesis and tumor progression (J. Cell Physiol. 2009;223:519-529; Cancer Biol. Ther. 2010;9:66-76). Here we have determined by immunohistochemistry the localization and cellular distribution of \u03B3-tubulin in clinical tissue samples from 109 non-small cell lung cancer (NSCLC) cases. The expression and distribution of \u03B3-tubulin protein and transcripts was also determined in the NSCLC tumor cell lines NCI-H460 (HTB-177) and NCI-H69 (HTB-119) by immunocytochemistry, quantitative immunoblotting and reverse transcription quantitative real-time PCR (RT-qPCR). Polyclonal and monoclonal anti-peptide antibodies recognizing epitopes in the C- or N-terminal domains of \u03B3-tubulins and human gene-specific primers for \u03B3-tubulins 1 (TUBG1) and 2 (TUBG2) were used. In non-neoplastic cells of the airway epithelium in situ, \u03B3-tubulin exhibited predominantly apical surface and pericentriolar localizations. In contrast, markedly increased, albeit heterogeneous and variously prominent \u03B3-tubulin immunoreactivity was detected in clinical tumor specimens and in the NCI-H460 and NCI-H69 cell lines, where tumor cells exhibited overlapping multi-punctate and diffuse patterns of localization. Co-expression of \u03B3-tubulin and Ki-67 (MIB-1) was detected in a population of proliferating tumor cells. A statistically significant increase of \u03B3-tubulin expression was found in Stage III compared to lesser stage tumors (p<0.001 v. Stages I/II) regardless of histological subtype or grade. By quantitative immunoblotting NCI-H460 and NCI-H69 cells expressed higher levels of \u03B3-tubulin protein compared to small airway epithelial cells (SAEC). In both tumor cell lines increase in TUBG1 and TUBG2 transcripts was detected by RT-qPCR. Our results reveal for the first time an increased expression of \u03B3-tubulin in lung cancer."@en . "000306521400007" . "Caracciolo, V." . . . . "157462" . "27" . "Overexpression of \u03B3-tubulin in non-small cell lung cancer" . . "Dr\u00E1berov\u00E1, Eduarda" . "Katsetos, C. D." . "Trakas, N." . . "Overexpression of \u03B3-tubulin in non-small cell lung cancer"@en . "Sulimenko, Tetyana" . . "Emmanouilidou, A." . . "Mahera, E." . . "12"^^ . "Maounis, N. F." . "ES - \u0160pan\u011Blsk\u00E9 kr\u00E1lovstv\u00ED" . "9" . . "RIV/68378050:_____/12:00387840" . . "RIV/68378050:_____/12:00387840!RIV13-GA0-68378050" . "Overexpression of \u03B3-tubulin in non-small cell lung cancer" . "Chorti, M." . "P(GA204/09/1777), P(GAP302/10/1701), P(LC545), Z(AV0Z50520514)" . . . . "0213-3911" . "3"^^ . "Dr\u00E1ber, Pavel" . "Histology and Histopathology" . . . "Overexpression of \u03B3-tubulin in non-small cell lung cancer"@en .