. "\u010Cerm\u00E1kov\u00E1, Kate\u0159ina" . . . . "RIV/68378050:_____/12:00376532" . . "Arabinose repressor AraR in Bacillus subtilis negatively controls expression of genes in the metabolic pathway of arabinose containing polysaccharides. The protein is composed of two domains of different phylogenetic origin and function: the N-terminal DNA binding domain belonging to GntR family and the C-terminal effector binding domain, a GalR/LacI family member. We determined the crystal structure of the C-terminal effector-binding domain of AraR in complex with the effector L-arabinose at 2.2 angstrom resolution. The L-arabinose binding affinity was characterized by isothermal titration calorimetry and differential scanning fluorimetry, the Kd value was 8.4 +/- 0.4 microM. Effect of L-arabinose on the protein oligomeric state was investigated in a solution and a detailed analysis of crystal identified dimer organization distinctive from other members of the GalR/LacI family." . . "Arabinose repressor AraR in Bacillus subtilis negatively controls expression of genes in the metabolic pathway of arabinose containing polysaccharides. The protein is composed of two domains of different phylogenetic origin and function: the N-terminal DNA binding domain belonging to GntR family and the C-terminal effector binding domain, a GalR/LacI family member. We determined the crystal structure of the C-terminal effector-binding domain of AraR in complex with the effector L-arabinose at 2.2 angstrom resolution. The L-arabinose binding affinity was characterized by isothermal titration calorimetry and differential scanning fluorimetry, the Kd value was 8.4 +/- 0.4 microM. Effect of L-arabinose on the protein oligomeric state was investigated in a solution and a detailed analysis of crystal identified dimer organization distinctive from other members of the GalR/LacI family."@en . "Structure of the effector-binding domain of the arabinose repressor AraR from Bacillus subtilis"@en . . "DK - D\u00E1nsk\u00E9 kr\u00E1lovstv\u00ED" . "P(ME08016), Z(AV0Z40550506), Z(AV0Z50520514)" . . "Pachl, Petr" . "Otwinowski, Z." . "RIV/68378050:_____/12:00376532!RIV13-AV0-68378050" . . "Structure of the effector-binding domain of the arabinose repressor AraR from Bacillus subtilis" . . . . "000299469100011" . "repressor; dimerization; effector binding; isothermal titration calorimetry"@en . . "171899" . "\u0158ez\u00E1\u010Dov\u00E1, Pavl\u00EDna" . "0907-4449" . "Structure of the effector-binding domain of the arabinose repressor AraR from Bacillus subtilis"@en . . . "F\u00E1bry, Milan" . "2"^^ . "2" . "Structure of the effector-binding domain of the arabinose repressor AraR from Bacillus subtilis" . . "[79AB189B244E]" . "10.1107/S090744491105414X" . "Proch\u00E1zkov\u00E1, Kate\u0159ina" . . "7"^^ . . "68" . "Sieglov\u00E1, Irena" . "10"^^ . . . "Acta Crystallographica Section D-Biological Crystallography" . .