"Specifick\u00E9 monoklon\u00E1ln\u00ED protil\u00E1tky proti CPSF6 (cleavage and polyadenylation specificity factor subunit 6) byly p\u0159ipraveny imunizac\u00ED Balb/C my\u0161\u00ED sm\u011Bs\u00ED skelet\u00E1ln\u00EDch protein\u016F z bun\u011Bk HeLa. Protil\u00E1tky byly nav\u00E1z\u00E1ny na nov\u00E9 zlat\u00E9 nano\u010D\u00E1stice ty\u010Dinkovit\u00E9ho tvaru o velikosti 18 nm. Tyto konjug\u00E1ty jsou pou\u017Eiteln\u00E9 pro p\u0159\u00EDmou imunodetekci CK18 pomoc\u00ED elektronov\u00E9 mikroskopie. Specifick\u00FD tvar a prvkov\u00E9 slo\u017Een\u00ED nano\u010D\u00E1stic umo\u017E\u0148uje jejich pou\u017Eit\u00ED v kombinaci s jin\u00FDmi dostupn\u00FDmi nano\u010D\u00E1sticemi pro \u00FA\u010Dely v\u00EDcen\u00E1sobn\u00E9ho zna\u010Den\u00ED antigen\u016F v biologick\u00FDch vzorc\u00EDch. Konjug\u00E1ty jsou d\u016Fle\u017Eit\u00FDm n\u00E1strojem pou\u017Eiteln\u00FDm pro detekci a funk\u010Dn\u00ED charakterizaci proteinu CPSF6 jako clenu skupiny gen\u016F souvisej\u00EDc\u00EDch s regulac\u00ED metastatick\u00E9ho a invazivn\u00EDho potenci\u00E1lu \u0159ady zhoubn\u00FDch n\u00E1dor\u016F."@cs . "Mouse monoclonal antibody to CPSF6 (cleavage and polyadenylation specificity factor subunit 6; clone 3B6.0.C7) conjugated with gold rod-shaped nanoparticles"@en . "my\u0161\u00ED monoklon\u00E1ln\u00ED protil\u00E1tka proti antigenu CPSF6 (cleavage and polyadenylation specificity factor subunit 6; klon 3B6.0.C7) nav\u00E1zan\u00E1 na zlat\u00E9 nanoty\u010Dinky" . . . . "Mouse monoclonal antibody to CPSF6 (cleavage and polyadenylation specificity factor subunit 6; clone 3B6.0.C7) conjugated with gold rod-shaped nanoparticles"@en . . "RIV/68378050:_____/11:00372719" . . . . "My\u0161\u00ED monoklon\u00E1ln\u00ED protil\u00E1tka proti antigenu CPSF6 (cleavage and polyadenylation specificity factor subunit 6; klon 3B6.0.C7) nav\u00E1zan\u00E1 na zlat\u00E9 nanoty\u010Dinky"@cs . "Specifick\u00E9 monoklon\u00E1ln\u00ED protil\u00E1tky proti CPSF6 (cleavage and polyadenylation specificity factor subunit 6) byly p\u0159ipraveny imunizac\u00ED Balb/C my\u0161\u00ED sm\u011Bs\u00ED skelet\u00E1ln\u00EDch protein\u016F z bun\u011Bk HeLa. Protil\u00E1tky byly nav\u00E1z\u00E1ny na nov\u00E9 zlat\u00E9 nano\u010D\u00E1stice ty\u010Dinkovit\u00E9ho tvaru o velikosti 18 nm. Tyto konjug\u00E1ty jsou pou\u017Eiteln\u00E9 pro p\u0159\u00EDmou imunodetekci CK18 pomoc\u00ED elektronov\u00E9 mikroskopie. Specifick\u00FD tvar a prvkov\u00E9 slo\u017Een\u00ED nano\u010D\u00E1stic umo\u017E\u0148uje jejich pou\u017Eit\u00ED v kombinaci s jin\u00FDmi dostupn\u00FDmi nano\u010D\u00E1sticemi pro \u00FA\u010Dely v\u00EDcen\u00E1sobn\u00E9ho zna\u010Den\u00ED antigen\u016F v biologick\u00FDch vzorc\u00EDch. Konjug\u00E1ty jsou d\u016Fle\u017Eit\u00FDm n\u00E1strojem pou\u017Eiteln\u00FDm pro detekci a funk\u010Dn\u00ED charakterizaci proteinu CPSF6 jako clenu skupiny gen\u016F souvisej\u00EDc\u00EDch s regulac\u00ED metastatick\u00E9ho a invazivn\u00EDho potenci\u00E1lu \u0159ady zhoubn\u00FDch n\u00E1dor\u016F." . "My\u0161\u00ED monoklon\u00E1ln\u00ED protil\u00E1tka proti antigenu CPSF6 (cleavage and polyadenylation specificity factor subunit 6; klon 3B6.0.C7) nav\u00E1zan\u00E1 na zlat\u00E9 nanoty\u010Dinky" . "1"^^ . "Specific monoclonal antibodies to CPSF6 (cleavage and polyadenylation specificity factor subunit 6; klon 3B6.0.C7) were obtained by immunization of Balb/C mice with the mixture of skeletal proteins from HeLa cells. The antibodies were conjugated with novel gold rod-shaped 18-nm nanoparticles. These conjugates are useful for direct immunodetection of CPSF6 by electron microscopy. Specific shape and elemental composition of the nanoparticles makes it possible to use the conjugates for multiple immunolabeling of antigens in biological specimens. The conjugates represent an important tool for detection and functional characterization of CPSF6 as a protein belonging to a group of genes connected with the regulation of metastatic and invasive potential in various cancers."@en . . . "1"^^ . "P(KAN200520704), Z(AV0Z50520514)" . "realiz\u00E1tor Central European Biosystems s.r.o., I\u010CO: 27124762" . . . . . "214463" . "Hoz\u00E1k, Pavel" . "v\u00FDsledek p\u0159ipraven ke komer\u010Dn\u00EDmu vyu\u017Eit\u00ED" . . "My\u0161\u00ED monoklon\u00E1ln\u00ED protil\u00E1tka proti antigenu CPSF6 (cleavage and polyadenylation specificity factor subunit 6; klon 3B6.0.C7) nav\u00E1zan\u00E1 na zlat\u00E9 nanoty\u010Dinky"@cs . . . . . "CPSF6; monoclonal antibody; immunogold detection; cancer"@en . . "My\u0161\u00ED monoklon\u00E1ln\u00ED protil\u00E1tka proti antigenu CPSF6 (cleavage and polyadenylation specificity factor subunit 6; klon 3B6.0.C7) nav\u00E1zan\u00E1 na zlat\u00E9 nanoty\u010Dinky" . "RIV/68378050:_____/11:00372719!RIV12-AV0-68378050" . . . "3B6.0.C7-1" . "[07752270BDED]" . . .