"000294726800019" . . "0021-9258" . "DNA damage; DNA polymerase; DNA repair; DNA replication; DNA-protein interaction"@en . . "Le Gac, N.T." . "Syvaaja, J. E." . . . . "In vitro gap-directed translesion DNA synthesis of an abasic site involving human DNA polymerases epsilon, lambda, and beta"@en . . "Gironis, N." . . "1"^^ . . "Z(AV0Z50520514)" . "204041" . "37" . "RIV/68378050:_____/11:00372429" . "DNA polymerase (pol) \u03F5 is thought to be the leading strand replicase in eukaryotes, whereas pols \u03BB and \u03B2 are believed to be mainly involved in re-synthesis steps of DNA repair. DNA elongation by the human pol \u03F5 is halted by an abasic site (apurinic/apyrimidinic (AP) site). In this study, we present in vitro evidence that human pols \u03BB, \u03B2, and \u03B7 can perform translesion synthesis (TLS) of an AP site in the presence of pol \u03F5, likely by initiating the 3OHs created at the lesion by the arrested pol \u03F5. However, in the case of pols \u03BB and \u03B2, this TLS requires the presence of a DNA gap downstream from the product synthesized by the pol \u03F5, and the optimal gap for efficient TLS is different for the two polymerases. Collectively, our in vitro results support the existence of a mechanism of gap-directed TLS at an AP site involving a switch between the replicative pol \u03F5 and the repair pols \u03BB and \u03B2." . . "H\u00FCbscher, U." . "di Cicco, G." . . "[D66EF6A60C10]" . "Pospiech, H." . . "10"^^ . "DNA polymerase (pol) \u03F5 is thought to be the leading strand replicase in eukaryotes, whereas pols \u03BB and \u03B2 are believed to be mainly involved in re-synthesis steps of DNA repair. DNA elongation by the human pol \u03F5 is halted by an abasic site (apurinic/apyrimidinic (AP) site). In this study, we present in vitro evidence that human pols \u03BB, \u03B2, and \u03B7 can perform translesion synthesis (TLS) of an AP site in the presence of pol \u03F5, likely by initiating the 3OHs created at the lesion by the arrested pol \u03F5. However, in the case of pols \u03BB and \u03B2, this TLS requires the presence of a DNA gap downstream from the product synthesized by the pol \u03F5, and the optimal gap for efficient TLS is different for the two polymerases. Collectively, our in vitro results support the existence of a mechanism of gap-directed TLS at an AP site involving a switch between the replicative pol \u03F5 and the repair pols \u03BB and \u03B2."@en . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "10.1074/jbc.M111.246611" . "Villani, G." . "RIV/68378050:_____/11:00372429!RIV12-AV0-68378050" . "In vitro gap-directed translesion DNA synthesis of an abasic site involving human DNA polymerases epsilon, lambda, and beta"@en . . "286" . . "Parkkinen, S." . "Shevelev, Igor" . "In vitro gap-directed translesion DNA synthesis of an abasic site involving human DNA polymerases epsilon, lambda, and beta" . "Markennen, E." . . . "11"^^ . . "Journal of Biological Chemistry" . "In vitro gap-directed translesion DNA synthesis of an abasic site involving human DNA polymerases epsilon, lambda, and beta" . "Shevelev, Igor" . .