"Various resins (Lowicryl, LR White, LR Gold, Unicryl) used for embedding were compared for their ability to reveal antigens for immunogold labelling on their surface. Their surfaces after cutting with a diamond knife were observed using atomic force microscopy, and results statistically analyzed. The LR White resin in combination with HPF/FS was shown to be able to be successfully used for fine ultrastructural immunocytochemistry. Methods were presented allowing detection of clustering or colocalization of antigens/gold particles. A complete set of tools is now available for reliable detection of gold particles in EM images, their statistical evaluation and production of convenient outputs for publication of results. The plug-ins are useful addition to image analysis software accompanying modern CCD cameras for electron microscopes." . . . "Optimalization of immunogold labeling on thin resin sections after cryofixation"@en . "P(2B06063), P(GD204/09/H084), P(KAN200520704), P(LC545), Z(AV0Z40400503), Z(AV0Z50110509), Z(AV0Z50520514)" . "[FCF0508FCCAE]" . "8"^^ . . . . . . "Graz" . . "Graz" . . . "331969" . . "Philimonenko, Anatolij" . "Optimalization of immunogold labeling on thin resin sections after cryofixation" . . . . "cell ultrastructure; high pressure freezing; freeze substitutions; resins; immunocytochemistry"@en . "Optimalization of immunogold labeling on thin resin sections after cryofixation" . . "Janda, Pavel" . . . . . . . "2"^^ . . "RIV/68378050:_____/09:00336266!RIV10-MSM-68378050" . "978-3-85125-062-6" . . . "MC 2009 - Microscopy Conference: First Joint Meeting of Dreil\u00E4ndertagung and Multinational Conference on Microscopy" . "Jan\u00E1\u010Dek, Ji\u0159\u00ED" . "Hoz\u00E1k, Pavel" . "RIV/68378050:_____/09:00336266" . "Vyhnal, Ale\u0161" . "2009-08-30+02:00"^^ . "Sobol, Margaryta" . "Philimonenko, Vlada" . "Optimalization of immunogold labeling on thin resin sections after cryofixation"@en . "Various resins (Lowicryl, LR White, LR Gold, Unicryl) used for embedding were compared for their ability to reveal antigens for immunogold labelling on their surface. Their surfaces after cutting with a diamond knife were observed using atomic force microscopy, and results statistically analyzed. The LR White resin in combination with HPF/FS was shown to be able to be successfully used for fine ultrastructural immunocytochemistry. Methods were presented allowing detection of clustering or colocalization of antigens/gold particles. A complete set of tools is now available for reliable detection of gold particles in EM images, their statistical evaluation and production of convenient outputs for publication of results. The plug-ins are useful addition to image analysis software accompanying modern CCD cameras for electron microscopes."@en . "4"^^ . . . . "Verlag der Technischen Universit\u00E4t" . "Kub\u00EDnov\u00E1, Lucie" . .