. . . . "In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer" . "RIV/68378050:_____/09:00334099" . . . . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer" . "318932" . . "RIV/68378050:_____/09:00334099!RIV10-MSM-68378050" . "6"^^ . "Expression of the nascent RNA transcript is regulated by its interaction with a number of proteins. Misregulation of such interactions often results in impaired cellular functions that can lead to cancer and many diseases. Our understanding of RNA\u2013protein interaction within the cellular context is thus essential for development of novel diagnostic and therapeutic tools. While there are many in vitro methods analyzing RNA\u2013protein interactions, in vivo approaches are scarce. Here we established a method based on fluorescence resonance energy transfer (FRET), which we termed RNA-binding mediated FRET (RB-FRET). The methods enables determination of RNA\u2013protein interaction inside cells and was tested on hnRNP H protein binding to its cognate RNA. Using two different approaches, we provided evidence that RB-FRET was sensitive enough to detect specific RNA\u2013protein interactions in cell, providing a powerful tool to study spatial and temporal localization of specific RNA\u2013protein complexes."@en . "15" . . . "Expression of the nascent RNA transcript is regulated by its interaction with a number of proteins. Misregulation of such interactions often results in impaired cellular functions that can lead to cancer and many diseases. Our understanding of RNA\u2013protein interaction within the cellular context is thus essential for development of novel diagnostic and therapeutic tools. While there are many in vitro methods analyzing RNA\u2013protein interactions, in vivo approaches are scarce. Here we established a method based on fluorescence resonance energy transfer (FRET), which we termed RNA-binding mediated FRET (RB-FRET). The methods enables determination of RNA\u2013protein interaction inside cells and was tested on hnRNP H protein binding to its cognate RNA. Using two different approaches, we provided evidence that RB-FRET was sensitive enough to detect specific RNA\u2013protein interactions in cell, providing a powerful tool to study spatial and temporal localization of specific RNA\u2013protein complexes." . . "In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer"@en . . "[DC128D3C8B2E]" . "2"^^ . "R N A" . . . "000270851000014" . "9"^^ . "Jablonski, J. A" . "In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer"@en . . "Huranov\u00E1, Martina" . "Benda, Ale\u0161" . . "P(KAN200520801), P(LC06063), Z(AV0Z40400503), Z(AV0Z50520514)" . "1355-8382" . . "Stan\u011Bk, David" . . "11" . "FRET; FLIM; RNA-protein interactions"@en . "Hof, Martin" . . "Caputi, M." .