. . . . "21" . . . "000267099800011" . "Vacuolin-1-modulated exocytosis and cell resealing in mast cells" . . . . . "Heneberg, Petr" . . . "RIV/68378050:_____/09:00334054!RIV10-MSM-68378050" . "Dr\u00E1berov\u00E1, Lubica" . "[B6E753D6A6B6]" . "8" . . "Vacuolin-1-modulated exocytosis and cell resealing in mast cells"@en . . "Vacuolin-1-modulated exocytosis and cell resealing in mast cells" . "vacuolin-1; exocytosis; mast cells"@en . "Dr\u00E1ber, Petr" . "Vacuolin-1-modulated exocytosis and cell resealing in mast cells"@en . "4"^^ . . "The small chemical vacuolin-1 induces rapid formation of large vacuoles in various cell types. In epithelial cells, vacuolin-1 has been shown to inhibit Ca2+ ionophore-induced exocytosis depending on experimental conditions used but had no effect on repair of damaged membranes. However, it is not known whether vacuolin-1 could inhibit exocytosis induced by immunoreceptor triggering in professional secretory cells and whether there is any correlation between effect of vacuolin-1 on exocytosis and membrane repair in such cells. Here we show that in rat basophilic leukemia (RBL-2H3) cells activated by the high-affinity IgE receptor (FcepsilonRI) triggering vacuolin-1 enhanced exocytosis. Under identical conditions of activation, vacuolin-1 inhibited exocytosis in mouse bone marrow-derived mast cells. Our data indicate that lysosomal exocytosis exhibits different sensitivity to vacuolin-1 depending on the cell type analyzed and mode of activation."@en . "348336" . "9"^^ . . "4"^^ . . "The small chemical vacuolin-1 induces rapid formation of large vacuoles in various cell types. In epithelial cells, vacuolin-1 has been shown to inhibit Ca2+ ionophore-induced exocytosis depending on experimental conditions used but had no effect on repair of damaged membranes. However, it is not known whether vacuolin-1 could inhibit exocytosis induced by immunoreceptor triggering in professional secretory cells and whether there is any correlation between effect of vacuolin-1 on exocytosis and membrane repair in such cells. Here we show that in rat basophilic leukemia (RBL-2H3) cells activated by the high-affinity IgE receptor (FcepsilonRI) triggering vacuolin-1 enhanced exocytosis. Under identical conditions of activation, vacuolin-1 inhibited exocytosis in mouse bone marrow-derived mast cells. Our data indicate that lysosomal exocytosis exhibits different sensitivity to vacuolin-1 depending on the cell type analyzed and mode of activation." . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . "RIV/68378050:_____/09:00334054" . . . . . "Cellular Signalling" . "0898-6568" . "P(1M0506), P(GA301/09/1826), P(LC545), Z(AV0Z50520514), Z(MSM0021620814)" . "Shaik, Gouse Mohiddin" .