"P\u0159\u00EDprava rekombinantn\u00EDho proteinu Synaptotagminu 7."@cs . . . "Realiz\u00E1tor EXBIO Praha, a.s., Nad Safinou II 366, 252 42 Vestec" . "Preparation of recombinant protein Synaptotagmin 7"@en . . . . . "\u010Cern\u00FD, J." . "P\u0159\u00EDprava rekombinantn\u00EDho proteinu Synaptotagminu 7." . . . . "[C421F3368BF0]" . "2"^^ . . "Purifikovan\u00FD rekombinantn\u00ED protein Synaptotagminu 7" . . "Byly navr\u017Eeny \u010Dty\u0159i sady sady primer\u016F s restrik\u010Dn\u00EDmi m\u00EDsty pro restrikt\u00E1zy BamHI a XhoI pro p\u0159\u00EDpravu \u010Dty\u0159 reprezentativn\u00EDch fragment\u016F Syt7, dostate\u010Dn\u011B odli\u0161n\u00FDch od ostatn\u00EDch fosfodiester\u00E1z. Fragmenty byly p\u0159es restrik\u010Dn\u00ED m\u00EDsta XhoI/BamHI vlo\u017Eeny do expresn\u00EDho vektoru pGEX- 2TL, kter\u00FD nese sekvenci pro GST tag a byly exprimov\u00E1ny v bu\u0148k\u00E1ch E. coli BL21/ (\u03BBDE3) pLys. V\u00FDsledn\u00FD protein byl purifikov\u00E1n standardn\u00EDmi metodami v mno\u017Estv\u00ED dostate\u010Dn\u00E9m pro imunizaci."@cs . . "1"^^ . . "Preparation of recombinant protein Synaptotagmin 7"@en . "Byly navr\u017Eeny \u010Dty\u0159i sady sady primer\u016F s restrik\u010Dn\u00EDmi m\u00EDsty pro restrikt\u00E1zy BamHI a XhoI pro p\u0159\u00EDpravu \u010Dty\u0159 reprezentativn\u00EDch fragment\u016F Syt7, dostate\u010Dn\u011B odli\u0161n\u00FDch od ostatn\u00EDch fosfodiester\u00E1z. Fragmenty byly p\u0159es restrik\u010Dn\u00ED m\u00EDsta XhoI/BamHI vlo\u017Eeny do expresn\u00EDho vektoru pGEX- 2TL, kter\u00FD nese sekvenci pro GST tag a byly exprimov\u00E1ny v bu\u0148k\u00E1ch E. coli BL21/ (\u03BBDE3) pLys. V\u00FDsledn\u00FD protein byl purifikov\u00E1n standardn\u00EDmi metodami v mno\u017Estv\u00ED dostate\u010Dn\u00E9m pro imunizaci." . . "P\u0159\u00EDprava rekombinantn\u00EDho proteinu Synaptotagminu 7."@cs . "Ho\u0159ej\u0161\u00ED, V\u00E1clav" . . "RIV/68378050:_____/08:00323965!RIV09-AV0-68378050" . . "Synaptotagmin 7; recombinant protein; immunization"@en . . "389501" . "Four sets of primers with restriction sites for restrictases BamHI a XhoI were designed for preparation of of four representative fragments of Syt7, sufficiently different from other phosphodiesterases. The fragments were inserted via the XhoI/BamHI restriction sites into expression vectoru pGEX- 2TL carrying the sequence for GST tag and were expressed in cells E. coli BL21/ (lambdaDE3) pLys. The protein produced was purified by standard methods in the amounts sufficient for immunization."@en . "P(1M0506), Z(AV0Z50520514)" . "Syt7" . . "P\u0159\u00EDprava rekombinantn\u00EDho proteinu Synaptotagminu 7." . . . "V\u00FDsledek p\u0159ipraven ke komer\u010Dn\u00EDmu vyu\u017Eit\u00ED" . "RIV/68378050:_____/08:00323965" . .